Chemical Profile and In Vitro Evaluation of the Antibacterial Activity of Dioscorea communis Berry Juice

Sci

Jörg Heilmann¹  Michail Christou Rallis²  George Ladopoulos³  Helen Skaltsa³  Christina Barda³  Maria-Eleni Grafakou³  Konstantina Tsami³  Dimitris Mossialos⁴  Nikos Asoutis Didaras⁴ 

[1] Department of Pharmaceutical Biology, Faculty of Pharmacy and Chemistry, University of Regensburg, D-93053 Regensburg, Germany;Department of Pharmaceutical Technology, Unit of Dermatopharmacology, Faculty of Pharmacy, School of Health Sciences, National & Kapodistrian University of Athens, Zografou, 15771 Athens, Greece;Department of Pharmacognosy & Chemistry of Natural Products, Faculty of Pharmacy, School of Health Sciences, National & Kapodistrian University of Athens, Zografou, 15771 Athens, Greece;Laboratory of Microbial Biotechnology-Molecular Bacteriology-Virology, Department of Biochemistry & Biotechnology, Faculty of Health Sciences, University of Thessaly, 41500 Larissa, Greece;

关键词: Dioscorea communis;    Dioscoreaceae;    GC-MC;    LC-MS;    NMR;    antibacterial;   

DOI  :  10.3390/sci4020021

来源: DOAJ

【 摘 要 】

Within the large family of Dioscoreaceae, Dioscorea communis (L.) Caddick & Wilkin (syn. Tamus communis L.) is considered among the four most widespread representatives in Europe, and it is commonly known under the name black bryony or bryonia. To date, reports have revealed several chemical components from the leaves and tubers of this plant. Nevertheless, an extensive phytochemical investigation has not been performed on its berry juice. In the present study, metabolite profiling procedures, using LC-MS, GC-MS, and NMR approaches, were applied to investigate the chemical profile of the D. communis berries. This work reveals the presence of several metabolites belonging to different phytochemical groups, such as fatty acid esters, alkylamides, phenolic derivatives, and organic acids, with lactic acid being predominant. In parallel, based on orally transmitted traditional uses, the initial extract and selected fractions were tested in vitro for their antibacterial effects and exhibited good activity against two bacterial strains related to skin infections: methicillin-resistant Staphylococcus aureus and Cutibacterium acnes. The MIC and MBC values of the extract were determined at 1.56% w/v against both bacteria. The results of this study provide important information on the chemical characterization of the D. communis berry juice, unveiling the presence of 71 metabolites, which might contribute to and further explain its specific antibacterial activity and its occasional toxicity.

【 授权许可】

Unknown