The Combination of Fasting, Acute Resistance Exercise, and Protein Ingestion Led to Different Responses of Autophagy Markers in Gastrocnemius and Liver Samples

Nutrients

Elisa M. B. Cabrera¹  Kellen C. C. Rodrigues²  Dennys E. Cintra²  Leandro P.de Moura²  Eduardo R. Ropelle²  José R. Pauli²  Tales S. Vieira³  Ellen C.de Freitas³  Gabriela Batitucci³  Alisson L.da Rocha⁴  Ana P. Pinto⁴  Adelino S. R.da Silva⁴  Eike B. Kohama⁴  Bruno B. Marafon⁵ 

[1] Institute of Translational Nutrigenetics and Nutrigenomics, Department of Molecular Biology and Genomics, Health Sciences University Center, University of Guadalajara, Guadalajara 44100, Mexico;Laboratory of Molecular Biology of Exercise (LaBMEx), School of Applied Sciences, University of Campinas (UNICAMP), Limeira, São Paulo 13484-350, Brazil;Postgraduate Program in Nutritional Science, State University of São Paulo Júlio de Mesquita Filho (Araraquara). Araraquara, São Paulo 14800-903, Brazil;Postgraduate Program in Rehabilitation and Functional Performance, Ribeirão Preto Medical School, University of São Paulo (USP), Ribeirão Preto, São Paulo 14049-900, Brazil;School of Physical Education and Sport of Ribeirão Preto, University of São Paulo (USP), Ribeirão Preto, São Paulo 14040-907, Brazil;

关键词: resistance exercise;    autophagy;    leucine;    mammalian target of rapamycin (mtor);    liver;    muscle;   

DOI  :  10.3390/nu12030641

来源: DOAJ

【 摘 要 】

The present study verified the responses of proteins related to the autophagy pathway after 10 h of fast with resistance exercise and protein ingestion in skeletal muscle and liver samples. The rats were distributed into five experimental groups: control (CT; sedentary and without gavage after fast), exercise immediately (EXE-imm; after fast, rats were submitted to the resistance protocol and received water by gavage immediately after exercise), exercise after 1 h (EXE-1h; after fast, rats were submitted to the resistance protocol and received water by gavage 1 h after exercise), exercise and supplementation immediately after exercise (EXE/Suppl-imm; after fast, rats were submitted to the resistance protocol and received a mix of casein: whey protein 1:1 (w/w) by gavage immediately after exercise), exercise and supplementation 1 h after exercise (EXE/Suppl-1h; after fast, rats were submitted to the resistance protocol and received a mix of casein: whey protein 1:1 (w/w) by gavage 1 h after exercise). In summary, the current findings show that the combination of fasting, acute resistance exercise, and protein blend ingestion (immediately or 1 h after the exercise stimulus) increased the serum levels of leucine, insulin, and glucose, as well as the autophagy protein contents in skeletal muscle, but decreased other proteins related to the autophagic pathway in the liver. These results deserve further mechanistic investigations since athletes are combining fasting with physical exercise to enhance health and performance outcomes.

【 授权许可】

Unknown