Antidiabetic and antiplatelet aggregation study of various methanol fractions of Nymphaea stellata Willd. leaves

Herba Polonica

Tamboli Riyaj Shaukat¹  Mishra Shri Hari²  Agilandeswari Devarajan³  Raja Mohan Maruga⁴ 

[1] Department of Herbal Drug Technology Faculty of Pharmacy The Maharaja Sayajirao University of Baroda G H Patel Building, Donor’s Plaza, Fatehgunj Vadodara-390002 Gujarat, India;Department of Herbal Drug Technology Faculty of Pharmacy The Maharaja Sayajirao University of Baroda G H Patel Building, Donor’s Plaza, Fatehgunj Vadodara-390002, Gujarat, India;Department of Pharmacognosy and Phytochemistry Hillside College of Pharmacy and Research Centre Raghuvanahalli 9 Kanakapura Main Road Bengaluru-560062, Karnataka, India;Department of Pharmacognosy and Phytochemistry, Hillside College of Pharmacy and Research Centre, Raghuvanahalli 9, Kanakapura Main Road, Bengaluru-560062, Karnataka, India;

关键词: STZ-NAD;    PTP1B inhibition;    ADP induced;    co-TLC;    histopathology;   

DOI  :  10.1515/hepo-2017-0016

来源: DOAJ

【 摘 要 】

Introduction: Nymphaea stellata Willd. (Nymphaeaceae) is traditionally used for the treatment of diabetes. Alcohol extract of N. stellata leaves has been reported for hypoglycaemic activity. Objective: The aim of this study was to further investigate the different methanol fractions of N. stellata leaves for anti-diabetic activity and anti-platelet aggregation activity. Methods: Methanol extract was fractioned in to unsaponified petroleum ether fraction of methanol extract (UPFME), chloroform fraction of methanol extract (CFME) and residual fraction of methanol extract (RFME). All fractions were evaluated for in vivo anti-diabetic activity (STZ-NAD-induced rat model), in vitro anti-diabetic activity (PTP1B inhibition study) and anti-platelet aggregation activity. Results: UPFME showed significant changes in all studied parameters, compared to the diabetic control. UPFME also showed an IC50 value of 19.30±1.1 mg/ml and 13.11±0.7 μg/ml in PTP1B inhibition study and anti-platelet aggregation study, respectively. Conclusion: The study indicates that UPFME of N. stellata leaves exhibit anti-diabetic and anti-platelet aggregation activity.

【 授权许可】

Unknown