Integrated analysis of a ceRNA network reveals potential prognostic lncRNAs in gastric cancer

Cancer Medicine

Mingran Qi¹  Fan Li¹  Bingxin Yu²  Huiyuan Yu³ 

[1] Department of Pathogenobiology The Key Laboratory of Zoonosis Chinese Ministry of Education College of Basic MedicineJilin University Changchun Jilin China;Department of Ultrasound China‐Japan Union Hospital of Jilin University Changchun Jilin China;School of Public Health Jilin University Changchun Jilin China;

关键词: biomarkers;    ceRNA network;    gastric cancer;    long noncoding RNA;    risk score;   

DOI  :  10.1002/cam4.2760

来源: DOAJ

【 摘 要 】

Abstract Long noncoding RNAs (lncRNAs) have important biological functions as competing endogenous RNAs (ceRNAs) in tumors, yet the functions and regulatory mechanisms of lncRNA‐related ceRNAs in gastric cancer have not been fully elucidated. In this study, we constructed a lncRNA‐miRNA‐mRNA ceRNA network and identified potential lncRNA biomarkers in gastric cancer. Basing on the RNA profiles downloaded from The Cancer Genome Atlas (TCGA) platform, the gastric cancer‐specific differentially expressed lncRNAs, miRNAs, and mRNAs were screened for constructing a ceRNA network using bioinformatic tools. The enrichment analysis of the biological processes in Gene Ontology and the Kyoto Encyclopedia of Genes and Genomes pathways was performed on the ceRNA‐related DEmRNAs. According to the modularization of protein‐protein interaction (PPI) network, we extracted a ceRNA subnetwork and analyzed the correlation between the expression of the lncRNAs involved and specific clinical features of patients. Next, the expression of highly up‐regulated in liver cancer (HULC) and RP11‐314B1.2 showed significant changes in several pathological processes involved in gastric cancer, and nine lncRNAs were found to be correlated with the overall survival of patients with gastric cancer. Through the univariate and multivariate Cox regression analyses, two lncRNAs (LINC00106 and RP11‐999E24.3) were identified and utilized to establish a risk score model for assessing the prognosis of patients. The analysis results were also partially verified using quantitative real‐time PCR. The findings from this study indicate that HULC, RP11‐314B1.2, LINC00106, and RP11‐999E24.3 could be considered as potential therapeutic targets or prognostic biomarkers in gastric cancer, and provide a new perspective for cancer pathogenesis research.

【 授权许可】

Unknown