Molecular changes to tendons after collagenase-induced acute tendon injury in a senescence-accelerated mouse model

BMC Musculoskeletal Disorders

Yutaka Mifune¹  Atsuyuki Inui¹  Yasuhiro Ueda¹  Fumiaki Takase¹  Ryosuke Kuroda¹  Takashi Kurosawa¹  Takeshi Kataoka¹  Takeshi Kokubu¹  Kohei Yamaura¹ 

[1] Department of Orthopaedic Surgery, Kobe University Graduate School of Medicine;

关键词: Aging;    Acute tendon injury;    Tendon degeneration;   

DOI  :  10.1186/s12891-019-2488-1

来源: DOAJ

【 摘 要 】

Abstract Background Aging impairs tendon healing and is a potential risk factor for chronic tendinitis. During normal aging, tendons undergo structural and biomechanical degenerative changes, accompanied by a reduction in the number of tenocytes and changes to their properties. However, molecular changes in aged tendons under inflammatory conditions are not well understood. The present study analyzed the molecular changes in collagenase induced acute tendon injury using a senescence-accelerated mouse (SAM) model. Methods SAMP6 mice were used as an aging animal model and SAMR1 mice were used as a control to represent a senescence-resistant inbred strain. All the mice used in the study were 40 weeks old. Collagenase I from Clostridium histolyticum (20 μL) was injected percutaneously to the tendon-bone junction of the Achilles tendon. Two weeks after treatment, the Achilles tendons were harvested and stained using Picrosirius Red to determine collagen expression. Real-time PCR was performed to analyze gene expression of IL-6, tenomodulin, type I and type II collagen, MMP-9, TIMP-1, and TIMP-2. Results Collagenase injection resulted in significantly higher gene expression of IL-6 but significantly lower tenomodulin expression compared with the control in SAMP6 and SAMR1 mice. In SAMP6 mice, gene expression of type III collagen and MMP-9 was significantly higher in the collagenase-injected group compared with the control group. SAMP6 mice also showed lower expression of type I collagen, TIMP-1, and TIMP-2 in the collagenase-injected group compared with the control group. Picrosirius Red staining showed the highest expression of type III collagen in the collagenase-injected SAMP6 group compared with the other groups. Conclusions The collagenase-injected SAMP6 group showed higher expression of IL-6, MMP-9, and type III collagen and lower expression of type I collagen, TIMP-1, and TIMP-2, which are known to suppress metalloproteinases. The results indicate that aging may lead to dysfunction of the tendon healing process after acute tendon injury.

【 授权许可】

Unknown