In vivo and in vitro pharmacological activities of Tacca integrifolia rhizome and investigation of possible lead compounds against breast cancer through in silico approaches

Clinical Phytoscience

Md. Atiar Rahman¹  Farhana Binta Faiz²  Shahriar Ahmed²  Ahmed Rakib²  Bibi Humayra Khanam²  Mir Muhammad Nasir Uddin²  Md. Ashiqul Islam²  N. M. Mahmudul Alam Bhuiya³  Arkajyoti Paul⁴  Md. Nazim Uddin Chy⁴  Talha Bin Emran⁴  S. M. Amanat Ullah⁵ 

[1] Department of Biochemistry and Molecular Biology, University of Chittagong;Department of Pharmacy, Faculty of Biological Science, University of Chittagong;Department of Pharmacy, Jagannath University;Drug Discovery, GUSTO A Research Group;Medical Officer, Department of Haematology, Chittagong Medical College Hospital;

关键词: Tacca integrifolia;    Analgesic;    Anti-inflammatory;    Antipyretic;    PASS prediction;    Breast cancer;   

DOI  :  10.1186/s40816-019-0127-x

来源: DOAJ

【 摘 要 】

Abstract Background The study was conducted to evaluate the analgesic, anti-inflammatory, antipyretic properties of ethanolic extract of Tacca integrifolia rhizome (EETI) in mice and cytotoxic effects in brine shrimp nauplii followed by a PASS prediction study for some isolated compounds of T. integrifolia. Additionally, this experiment included the in silico molecular docking and ADME/T property analyses of some phytochemicals. Methods Formalin- induced paw licking test and acetic acid-induced writhing test for analgesic activity, carrageenan-induced paw edema test for anti-inflammatory potential and Brewer’s yeast-induced pyrexia test for antipyretic activity were applied. Antinociceptive and antineoplastic activity for breast cancer were revealed with PASS program. Schrodinger suite 2015 was used to evaluate the binding interaction and ADME/T properties of selected phytoconstituents with estrogen receptor alpha. Results In formalin-induced paw licking test, EETI at the doses of 200 and 400 mg/kg BW showed highly significant inhibition of writhing in both neurogenic and inflammatory phases. While EETI also exhibited highly significant, compared to control, writhing inhibition for both the doses in acetic acid-induced writhing test. Moderate anti-inflammatory effect at a dose of 400 mg/kg BW was noticed in paw-edema test. It also showed 77.51% of maximum antipyretic effect which was significantly effective compared to standard drug paracetamol (150 mg/kg) in Brewer’s yeast-induced pyrexia test. The EETI showed potential cytotoxic activity with LC50 value of 114.46 μg/mL. The PASS prediction revealed the potential antinociceptive and antineoplastic activity of target compounds. The compounds betulinic acid, quercetin-3-α-arabinoside, catsanogenin were found to be effective in molecular docking study. Conclusion It is evident that the EETI possesses highly significant analgesic activity with remarkable anti-inflammatory and antipyretic activity. The phytoconstituents have potential antinociceptive and antineoplastic (breast cancer) activity. Graphical abstract

【 授权许可】

Unknown