Applications of Time-Resolved Thermodynamics for Studies on Protein Reactions

J

Masahide Terazima¹ 

[1] Department of Chemistry, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan;

关键词: thermodynamics;    kinetics;    time resolved;    transient grating;    protein;    reaction;   

DOI  :  10.3390/j5010014

来源: DOAJ

【 摘 要 】

Thermodynamics and kinetics are two important scientific fields when studying chemical reactions. Thermodynamics characterize the nature of the material. Kinetics, mostly based on spectroscopy, have been used to determine reaction schemes and identify intermediate species. They are certainly important fields, but they are almost independent. In this review, our attempts to elucidate protein reaction kinetics and mechanisms by monitoring thermodynamic properties, including diffusion in the time domain, are described. The time resolved measurements are performed mostly using the time resolved transient grating (TG) method. The results demonstrate the usefulness and powerfulness of time resolved studies on protein reactions. The advantages and limitations of this TG method are also discussed.

【 授权许可】

Unknown