Effects of rumen-protected methionine and choline supplementation on vaginal discharge and uterine cytology of Holstein cows

International Journal of Veterinary Science and Medicine

Daniel N. Luchini¹  Zheng Zhou²  Cassandra S. Skenandore²  Maria I. Rivelli²  Diego A. Velasco Acosta²  Felipe C. Cardoso²  Márcio N. Corrêa³ 

[1] Adisseo NACA, 4400 North Point Parkway, Alpharetta, GA 30022, USA;Department of Animal Sciences, University of Illinois, 1207 West Gregory Dr., Urbana, IL 61801, USA;Federal University of Pelotas, Campus Universitário s/n, Capão do Leão, RS 96010-610, Brazil;

关键词: Choline;    Endometritis;    Holstein cows;    Methionine;    Metritis;    Polymorphonuclear neutrophils;   

DOI  :  10.1016/j.ijvsm.2017.05.001

来源: DOAJ

【 摘 要 】

Methionine is one of the most limiting amino acids in dairy diets and low feed intake around the time of calving could lead to decreased synthesis of phosphatidylcholine. An alternative pathway for phosphatidylcholine is to have choline as a precursor. The objective of this study was to determine the effects of feeding rumen-protected methionine and choline pre – and postpartum on reproduction of Holstein cows. Seventy-two Holstein cows were randomly assigned to four treatments from 21 days before calving to 30 days in milk (DIM): supplementation with rumen-protected methionine (MET; n = 20, received 0.08% of the dry matter (DM) of the diet/d as methionine, Smartamine M® to a Lys:Met = 2.9:1), rumen-protected choline (CHO; n = 17, received 60 g/d choline, Reassure), both rumen protected methionine and choline (MIX; n = 19, received 0.08% of the DM of the diet/d as methionine to a Lys:Met = 2.9:1 and 60 g/d choline), or no supplementation to serve as control (CON; n = 16, fed total mixed ration with a Lys:Met = 3.5:1). Cows were evaluated at 4, 7, 10, 13, 15, 17, and 30 d after calving for the presence of secretion using the Metricheck® device. On 15, 30, and 72 d after calving, the uterine endometrium of all cows was sampled using a cytological brush and streaked onto slides for analysis of the presence of polymorphonuclear neutrophils (PMN). We hypothesized that cows supplemented with methionine would have lower metricheck smell scores and lower rates of PMN than non-supplemented cows. On d 30, a treatment difference was detected using the metricheck score and smell (P < 0.04), with treatment MIX (score = 0.38) having a lower score than CHO (score = 2.11). Supplementing cows with rumen-protected methionine may have a beneficial effect on cows’ uterine health.

【 授权许可】

Unknown