(INVITED)Nanocoated fiber label-free biosensing for perfluorooctanoic acid detection by lossy mode resonance

Results in Optics

Francesco Chiavaioli¹  Ignacio Del Villar²  Ligia Maria Moretto³  Stefano Liberi⁴  Alessandro Angelini⁵  Giulia Moro⁶  Ambra Giannetti⁶  Francesco Baldini⁷  Karolien De Wael⁷  Pablo Zubiate⁸ 

[1] AXES Research Group, University of Antwerp, Groenenborgerlaan 171, 2020, Antwerp, Belgium;Corresponding author.;Institute of Smart Cities (ISC), Public University of Navarra, 31006 Pamplona, Spain;NanoLab Center of Excellence, University of Antwerp, Groenenborgerlaan 171, 2020 Antwerp, Belgium;Department of Biology, University of Padua, Via U. Bassi 58, 35131 Padova, Italy;Department of Molecular Sciences and Nanosystems, Ca’ Foscari University of Venice, Via Torino 155, 30172 Mestre, Italy;Electrical and Electronic Engineering Department, Public University of Navarra, 31006 Pamplona, Spain;Institute of Applied Physics “Nello Carrara”, National Research Council of Italy (CNR), 50019 Sesto Fiorentino, Firenze, Italy;

关键词: Nanocoated fiber sensor;    Lossy mode resonance;    Small target detection;    Delipidated human serum albumin;    Perfluorooctanoic acid;    Label-free biosensing;   

DOI  :  

来源: DOAJ

【 摘 要 】

The determination of per- and polyfluoroalkyl substances (PFAS) in environmental samples, such as drinking waters, requires the design of high performing and versatile sensing strategies. Label-free biosensing platforms based on specialty fiber optics are a valid option to face this challenge. Among them, lossy mode resonance (LMR) fiber optic biosensors are showing remarkable performance in terms of detection limit, selectivity, and reproducibility. The detection of small molecules, such as perfluorooctanoic acid (PFOA), can be achieved with the help of well-designed biological recognition layers. In this study, the biosensing potentialities of a label-free LMR-assisted optical platform based on nanocoated fibers are investigated. Delipidated human serum albumin (hSA) was used as biological recognition layer for PFOA in aqueous solution. Different fiber functionalization protocols based on the covalent immobilization of hSA were tested. The conformational changes related to the formation of hSA/PFOA complex were followed via optical monitoring of LMR spectral shift, showing a trend that can be modeled with Langmuir adsorption isotherm. These results confirmed the potentiality of LMR-based fiber biosensors for the detection of small molecules, such as PFOA, in synthetic samples.

【 授权许可】

Unknown