期刊论文详细信息
PeerJ
Heterologous expression of a Glyoxalase I gene from sugarcane confers tolerance to several environmental stresses in bacteria
Youxiong Que1  Yachun Su1  Qibin Wu1  Liping Xu1  Jinlong Guo1  Shiwu Gao1  Michael P. Grisham2  Yong-Bao Pan2 
[1] Fujian Agriculture and Forestry University, Key Laboratory of Sugarcane Biology and Genetic Breeding,Fuzhou, Fujian, China;USDA-ARS, Sugarcane Research Unit, Houma, LA, USA;
关键词: Functional validation;    SoGloI;    Expression analysis;    Stress tolerance;    Sugarcane;   
DOI  :  10.7717/peerj.5873
来源: DOAJ
【 摘 要 】

Glyoxalase I belongs to the glyoxalase system that detoxifies methylglyoxal (MG), a cytotoxic by-product produced mainly from triose phosphates. The concentration of MG increases rapidly under stress conditions. In this study, a novel glyoxalase I gene, designated as SoGloI was identified from sugarcane. SoGloI had a size of 1,091 bp with one open reading frame (ORF) of 885 bp encoding a protein of 294 amino acids. SoGloI was predicted as a Ni2+-dependent GLOI protein with two typical glyoxalase domains at positions 28–149 and 159–283, respectively. SoGloI was cloned into an expression plasmid vector, and the Trx-His-S-tag SoGloI protein produced in Escherichia coli was about 51 kDa. The recombinant E. coli cells expressing SoGloI compared to the control grew faster and tolerated higher concentrations of NaCl, CuCl2, CdCl2, or ZnSO4. SoGloI ubiquitously expressed in various sugarcane tissues. The expression was up-regulated under the treatments of NaCl, CuCl2, CdCl2, ZnSO4 and abscisic acid (ABA), or under simulated biotic stress conditions upon exposure to salicylic acid (SA) and methyl jasmonate (MeJA). SoGloI activity steadily increased when sugarcane was subjected to NaCl, CuCl2, CdCl2, or ZnSO4 treatments. Sub-cellular observations indicated that the SoGloI protein was located in both cytosol and nucleus. These results suggest that the SoGloI gene may play an important role in sugarcane’s response to various biotic and abiotic stresses.

【 授权许可】

Unknown   

  文献评价指标  
  下载次数:0次 浏览次数:0次