Astaxanthin reduces MMP expressions, suppresses cancer cell migrations, and triggers apoptotic caspases of in vitro and in vivo models in melanoma

Journal of Functional Foods

Chung-Yi Chen¹  Shi-Ying Huang²  Yen-Ting Chen³  Hsin-Yi Huang³  Hui-Min David Wang⁴  Yun-Sheng Lin⁵  Zhi-Hong Wen⁵  Chien-Jen Kao⁶ 

[1] Fujian Province key laboratory for the development of bioactive material from marine algae, Quanzhou, China;College of Oceanology and Food Science, Quanzhou Normal University, Quanzhou, China;Department of Fragrance and Cosmetic Science, Kaohsiung Medical University, Kaohsiung, Taiwan;Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung, Taiwan;Department of Nutrition and Health Science, School of Medical and Health Sciences, Fooyin University, Kaohsiung, Taiwan;National Defense Medical Center, Department of Internal medicine of Gangshan Branch of Kaohsiung Armed Forces General Hospital, Kaohsiung 82049, Taiwan;

关键词: Melanoma;    Astaxanthin;    Antioxidant;    In vivo;    Apoptosis;    Chemical compound studied in this article:;   

DOI  :  

来源: DOAJ

【 摘 要 】

This study assessed the use of astaxanthin as an anticancer agent for increasing inhibition to melanoma cells (A375 and A2058). Wound healing and invasion assays presented that astaxanthin treatment reduced melanoma cell migration in a dose-dependent manner. The effects on melanoma cell migration were conferred via suppressed expressions of matrix metalloproteinases 1, 2 and 9. Dichlorofluorescein diacetate assay further showed that astaxanthin treatment reduced production of cellular reactive oxygen species. Cellular proliferation assay revealed potent dose-dependent inhibiting effects on melanoma cells. One-dimensional flow cytometric analysis demonstrated that astaxanthin induced cell cycle arrest in G1 phase. Mechanisms of apoptosis were verified by double fluorescence staining with annexin V-fluorescein isothiocyanate and propidium iodide. The antitumor effects of astaxanthin significantly decreased tumor size in a xenograft model. In summary, the experimental results showed that astaxanthin has potent in vivo and in vitro inhibiting effects on melanoma tumor growth for developing as chemotherapeutic agents.

【 授权许可】

Unknown