A Sensitive and Selective Label-Free Electrochemical DNA Biosensor for the Detection of Specific Dengue VirusSerotype 3 Sequences

Sensors

Marli Cordeiro¹  Kennya Lopes²  Maria Amélia Borba³  José Lima-Filho³  Mariana Arruda³  Danyelly Martins³  Danielly Ferreira³  Deborah Zanforlin³  Wessulla Bezerra³  Natália Oliveira³  Gustavo Nascimento³  Elaine Souza⁴ 

[1] Departamento de Bioquímica, Universidade Federal de Pernambuco-UFPE,Av. Professor Moraes Rego, s/n, Campus da UFPE, CEP: 50670-901 Recife, PE, Brazil;Departamento de Virologia e Terapia Experimental (LAVITE), Centro de Pesquisas Aggeu Magalhães (CPqAM), Fundação Oswaldo Cruz (Fiocruz)—Pernambuco, Av. Professor Moraes Rego, s/n, Campus da UFPE, 50.670-420 Recife, PE, Brazil;Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco-UFPE, Av. Prof. Moraes Rego, s/n, Campus da UFPE, 50670-901 Recife, PE, Brazil;Universidade Federal de Alagoas (UFAL), Campus Arapiraca, Av. Manoel Severino Barbosa, s/n, Bom Sucesso, 57.309-005 Arapiraca, AL, Brazil;

关键词: dengue fever;    DNA biosensors;    differential pulse voltammetry;    electrochemical detection;    guanine oxidation;   

DOI  :  10.3390/s150715562

来源: DOAJ

【 摘 要 】

Dengue fever is the most prevalent vector-borne disease in the world, with nearly 100 million people infected every year. Early diagnosis and identification of the pathogen are crucial steps for the treatment and for prevention of the disease, mainly in areas where the co-circulation of different serotypes is common, increasing the outcome of dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). Due to the lack of fast and inexpensive methods available for the identification of dengue serotypes, herein we report the development of an electrochemical DNA biosensor for the detection of sequences of dengue virus serotype 3 (DENV-3). DENV-3 probe was designed using bioinformatics software and differential pulse voltammetry (DPV) was used for electrochemical analysis. The results showed that a 22-m sequence was the best DNA probe for the identification of DENV-3. The optimum concentration of the DNA probe immobilized onto the electrode surface is 500 nM and a low detection limit of the system (3.09 nM). Moreover, this system allows selective detection of DENV-3 sequences in buffer and human serum solutions. Therefore, the application of DNA biosensors for diagnostics at the molecular level may contribute to future advances in the implementation of specific, effective and rapid detection methods for the diagnosis dengue viruses.

【 授权许可】

Unknown