Quantifying How Staining Methods Bias Measurements of Neuron Morphologies

Frontiers in Neuroinformatics

Benjamin James Lansdell¹  Roozbeh Farhoodi²  Konrad Paul Kording³ 

[1] Department of Bioengineering, University of Pennsylvania, Philadelphia, PA, United States;Department of Mathematics, Sharif University of Technology, Tehran, Iran;Department of Neuroscience, University of Pennsylvania, Philadelphia, PA, United States;

关键词: dendritic morphology;    staining method;    rodent neuroanatomy;    neuroinformatics;    golgi method;    immunostaining;   

DOI  :  10.3389/fninf.2019.00036

来源: DOAJ

【 摘 要 】

The process through which neurons are labeled is a key methodological choice in measuring neuron morphology. However, little is known about how this choice may bias measurements. To quantify this bias we compare the extracted morphology of neurons collected from the same rodent species, experimental condition, gender distribution, age distribution, brain region and putative cell type, but obtained with 19 distinct staining methods. We found strong biases on measured features of morphology. These were largest in features related to the coverage of the dendritic tree (e.g., the total dendritic tree length). Understanding measurement biases is crucial for interpreting morphological data.

【 授权许可】

Unknown