Immune Response of an Oral Enterococcus faecalis Phage Cocktail in a Mouse Model of Ethanol-Induced Liver Disease

Viruses

Yi Duan¹  Beatriz Garcia Mendes¹  Bernd Schnabl¹ 

[1] Department of Medicine, University of California San Diego, La Jolla, San Diego, CA 92093, USA;

关键词: alcoholic hepatitis;    Enterococcus faecalis;    phage therapy;    immune response;    phage translocation;    host range;   

DOI  :  10.3390/v14030490

来源: DOAJ

【 摘 要 】

Cytolysin-positive Enterococcus faecalis (E. faecalis) cause more severe alcohol-associated hepatitis, and phages might be used to specifically target these bacteria in a clinical trial. Using a humanized mouse model of ethanol-induced liver disease, the effect of cytolytic E. faecalis phage treatment on the intestinal and liver immune response was evaluated. The observed immune response was predominantly anti-inflammatory and tissue-restoring. Besides, live phages could be readily recovered from the serum, spleen, and liver following oral gavage in ethanol-fed mice. We also isolated 20 new phages from the sewage water; six of them exhibited a relatively broad host range. Taken together, the oral administration of cytolytic E. faecalis phages leads to the translocation of phages to the systemic circulation and appears to be safe, following chronic-binge ethanol administration. A cocktail of three phages covers the majority of tested cytolysin-positive E. faecalis strains and could be tested in a clinical trial.

【 授权许可】

Unknown