CuO-NiO Nano composites: Synthesis, Characterization, and Cytotoxicity ‎evaluation ‎

Nanomedicine Research Journal

Farshid Keshavarzi¹  Yahya Azizi²  Mousa Aliahmad²  Abbas Rahdar³  Mahdiyeh Moudi⁴  Nasser Keikha⁴ 

[1] Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran;Department of Physics, Faculty of Science, University of Sistan and Baluchestan, Zahedan, Iran;Departments of Physics, Faculty of Science, University of Zabol, Zabol, Iran P.O.Box: 98155-987, Iran;Infectious Disease and Tropical Medicine Research Center, Zahedan University of Medical Sciences, Zahedan, Iran;

关键词: Nano-composites;    cytotoxicity;    Antifungal;   

DOI  :  10.22034/nmrj.2017.56956.1057

来源: DOAJ

【 摘 要 】

Objective(s): In this work, CuO- NiO nano-composites were synthesized via free-surfactant co-precipitation method and then their physiochemical properties, as well as cytotoxicity and antifungal effects, were studied. Methods: The structural and optical properties of CuO-NiO nanostructures were analyzed by X-ray diffraction (XRD), scanning electron microscopy (SEM), Atomic force microscope (AFM), UV–Vis absorption, and vibrating sample magnetometer (VSM) techniques. MTT assay was used to evaluate the cytotoxicity of nanostructures. Results: The cubical structure of CuO- NiO nano-composites was confirmed by the XRD technique. The optical study of the samples by UV-Vis indicted a blue shift in absorption wavelength with decreasing particle size due to quantum size effect. The super magnetic behavior of CuO-NiO nano composites after calcination was confirmed by magnetic characterization instrument. Finally, the results of cytotoxicity evaluation of CuO-NiO nano-composites at the lower concentrations on Breast cancer MDA cell lines demonstrate no significant toxicity. Minimum inhibitory concentration range and Minimum fungicidal concentration of nanoparticle were determined 0.97-15.62, 7.81µg/ml and for fluconazole were 1.75-25 µg/ml and 12.58 µg/ml, respectively. Conclusions: The study result of antimicrobialof CuO-NiO nano composites indicated an MIC90 antifungal activity with a concentration of 3.90µg/ml against vaginal isolates of C. albicans. The results of cytotoxicity study of nano-composites at concentration of 50µg/ml and 10µg/ml on the cell line of Breast cancer MDA was equivalent to %60 and %80, respectively.

【 授权许可】

Unknown