A Novel Rat Tail Needle Minimally Invasive Puncture Model Using Three-Dimensional Printing for Disk Degeneration and Progressive Osteogenesis Research

Frontiers in Cell and Developmental Biology

Liang Gao¹  Meijun Yan²  Wenhao Hu³  Xin Yin³  Baiwen Hu⁴  Xiaochuan Wu⁴  Jin Xiao⁴  Ting Ge⁴  Dongdong Xia⁴  Chi Zhang⁴  Jun Tan⁵  Junqi Liu⁶ 

[1] Center for Clinical Medicine, Hua Tuo Institute of Medical Innovation (HTIMI), Wuhan, China;Department of Orthopaedics, Shanghai General Hospital, Shanghai, China;Department of Orthopedics, Fourth Medical Center of PLA General Hospital, Beijing, China;Department of Orthopedics, Ningbo First Hospital, Ningbo, China;Department of Orthopedics, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai, China;Department of Radiotherapy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China;

关键词: disk degeneration;    rodent tail model;    vertebral hyperosteogeny;    minimally invasive procedure;    3D printing technique;   

DOI  :  10.3389/fcell.2021.587399

来源: DOAJ

【 摘 要 】

Many studies focused on the annulus fibrosus (AF) injury in rodent tail model for the intervertebral disk degeneration (IDD) research. However, previous studies caused tremendous injury of intervertebral disk (IVD) by penetrating whole disk. This study aimed to build a progressive IDD rodent tail model by a novel device for precise and minimally invasive puncture in AF. A precise puncture device was customized by 3D Printing Technique. 40 rodent tail IVDs were randomly grouped as follows: group A, non-puncture; group B, annulus needle puncture (ANP) for 4 week; group C, ANP for 8 week; and group D, ANP for 12 week. Pre- and post-puncture IVD height on radiographs and IVD signal intensity on T2 magnetic resonance imaging (MRI) were measured. Average bone density (ABD) on the end of coccygeal vertebrae between punctured disk was measured on the radiographs. Hematoxylin and eosin, TUNEL staining methods, immunofluorescence for cleaved-caspas3 and immunohistochemistry for aggrecan and collagen II were performed. Progressively and significantly increasing IVD height loss and degenerative grade were observed following the time points. The ABD was respectively, 81.20 ± 4.63 in group A, 83.93±3.18 in group B, 92.65 ± 4.32 in group C, 98.87 ± 6.69 in group D. In both group C and group D, there were significant differences with group A. In histology, increasing number of AF cells was noted in group B. In both group C and D, the fissures in AF were obviously observed, and a marked reduction of AF cells were also observed. In all ANP groups, there were significant decrease in number of NP cells, as well as aggrecan and collagen II contents. TUNEL assay showed cellular apoptosis were stimulated in all puncture group, especially in group D. A progressive IDD rat model could be standardly established by the micro-injury IVD puncture using a novel 3D printing device. This animal model provided a potential application for research of progressive hyperosteogeny following IDD development.

【 授权许可】

Unknown