Heterologous Biosynthesis of Artemisinin in Chrysanthemum morifolium Ramat

Separations

Svetlana Pigoleva¹  Sergey Dolgov¹  Aleksey Firsov¹  Alexander Pushin¹  Lyubov Shaloiko¹  Svetlana Motyleva²  Alexander Vainstein³ 

[1] Branch of the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Prospekt Nauki 6, 142290 Pushchino, Moscow Region, Russia;Federal Scientific Selection and Technology Center for Horticulture and Nursery, Zagoryevskaya St. 4, 115598 Moscow, Russia;Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel;

关键词: plant biotechnology;    malaria;    heterologous biosynthesis;    artemisinin;    Сhrysanthemum morifolium;    plant metabolic engineering;   

DOI  :  10.3390/separations8060075

来源: DOAJ

【 摘 要 】

Artemisinin-based drugs are the most effective medicine against multidrug-resistant Plasmodium spp., the parasite that causes malaria. To this day, wormwood A. annua L. is the sole commercial source of artemisinin, where it is produced in minor amounts. The artemisinin yield depends on numerous poorly regulated agricultural factors and the genetic variability of this non-domesticated plant. This has aroused significant interest in the development of heterologous expression platforms for artemisinin production. Previously, we obtained lines of Chrysanthemum morifolium Ramat. (C. morifolium Ramat.), cvs. White Snowdon and Egyptianka, transformed with artemisinin biosynthesis genes. Here, we report the results of an analysis of artemisinin production in transgenic chrysanthemums. Transcription of heterologous amorpha-4,11-diene monooxygenase and cytochrome P450 reductase genes in transgenic lines was confirmed using high-resolution melting analysis. Artemisinin accumulation was detected using GC-MS in White Snowdon plants, but not in Egyptianka ones, thereby demonstrating the possibility of transplanting active artemisinin biosynthetic pathway into chrysanthemum. Ways of increasing its content in producer plants are discussed.

【 授权许可】

Unknown