Evaluation of Transcriptomic Regulations behind Metabolic Syndrome in Obese and Lean Subjects

International Journal of Molecular Sciences

Magdalena Niemira¹  Agnieszka Bielska¹  Anna Citko¹  Magdalena Paczkowska-Abdulsalam¹  Anna Szałkowska¹  Adam Krętowski¹  Łukasz Szczerbiński¹  Edyta Adamska-Patruno¹  Katarzyna Maliszewska²  BeataAnna Raczkowska²  Sini Junttila³  Attila Gyenesei³ 

[1] Clinical Research Centre, Medical University of Białystok, M. Skłodowskiej-Curie 24A, 15-276 Białystok, Poland;Department of Endocrinology, Diabetology and Internal Medicine, Medical University of Białystok, M. Skłodowskiej-Curie 24A, 15-276 Białystok, Poland;Vienna Biocenter Core Facilities, Dr.-Bohr-Gasse 3, 1030 Vienna, Austria;

关键词: transcriptomics;    obesity;    obesity phenotypes;    metabolic syndrome;    cardiovascular disease;   

DOI  :  10.3390/ijms21041455

来源: DOAJ

【 摘 要 】

Multiple mechanisms have been suggested to confer to the pathophysiology of metabolic syndrome (MetS), however despite great interest from the scientific community, the exact contribution of each of MetS risk factors still remains unclear. The present study aimed to investigate molecular signatures in peripheral blood of individuals affected by MetS and different degrees of obesity. Metabolic health of 1204 individuals from 1000PLUS cohort was assessed, and 32 subjects were recruited to four study groups: MetS lean, MetS obese, “healthy obese”, and healthy lean. Whole-blood transcriptome next generation sequencing with functional data analysis were carried out. MetS obese and MetS lean study participants showed the upregulation of genes involved in inflammation and coagulation processes: granulocyte adhesion and diapedesis (p < 0.0001, p = 0.0063), prothrombin activation pathway (p = 0.0032, p = 0.0091), coagulation system (p = 0.0010, p = 0.0155). The results for “healthy obese” indicate enrichment in molecules associated with protein synthesis (p < 0.0001), mitochondrial dysfunction (p < 0.0001), and oxidative phosphorylation (p < 0.0001). Our results suggest that MetS is related to the state of inflammation and vascular system changes independent of excess body weight. Furthermore, “healthy obese”, despite not fulfilling the criteria for MetS diagnosis, seems to display an intermediate state with a lower degree of metabolic abnormalities, before they proceed to a full blown MetS.

【 授权许可】

Unknown