Facile Cell-Friendly Hollow-Core Fiber Diffusion-Limited Photofabrication

Frontiers in Bioengineering and Biotechnology

Alexander G. Savelyev¹  Natalya V. Sholina¹  Alina Yu. Kapitannikova¹  Tatiana N. Borodina²  Kirill V. Khaydukov²  Anton V. Mironov²  Andrei V. Zvyagin³  Anastasia V. Sochilina⁴  Roman A. Akasov⁴  Evgeny V. Khaydukov⁴  Alla N. Generalova⁴ 

[1] Center of Biomedical Engineering, Institute of Molecular Medicine, Sechenov University, Moscow, Russia;Federal Scientific Research Centre “Crystallography and Photonics” Russian Academy of Sciences, Moscow, Russia;MQ Photonics Centre, Faculty of Science and Engineering, Macquarie University, Sydney, NSW, Australia;Shemyakin-Ovchinnikov Institute of Bioorganic Сhemistry RAS, Moscow, Russia;

关键词: hyaluronic acid;    flavin mononucleotide;    hollow-core fiber;    cell-laden hydrogel;    photopolymerization;    photofabrication;   

DOI  :  10.3389/fbioe.2021.783834

来源: DOAJ

【 摘 要 】

Bioprinting emerges as a powerful flexible approach for tissue engineering with prospective capability to produce tissue on demand, including biomimetic hollow-core fiber structures. In spite of significance for tissue engineering, hollow-core structures proved difficult to fabricate, with the existing methods limited to multistage, time-consuming, and cumbersome procedures. Here, we report a versatile cell-friendly photopolymerization approach that enables single-step prototyping of hollow-core as well as solid-core hydrogel fibers initially loaded with living cells. This approach was implemented by extruding cell-laden hyaluronic acid glycidyl methacrylate hydrogel directly into aqueous solution containing free radicals generated by continuous blue light photoexcitation of the flavin mononucleotide/triethanolamine photoinitiator. Diffusion of free radicals from the solution to the extruded structure initiated cross-linking of the hydrogel, progressing from the structure surface inwards. Thus, the cross-linked wall is formed and its thickness is limited by penetration of free radicals in the hydrogel volume. After developing in water, the hollow-core fiber is formed with centimeter range of lengths. Amazingly, HaCaT cells embedded in the hydrogel successfully go through the fabrication procedure. The broad size ranges have been demonstrated: from solid core to 6% wall thickness of the outer diameter, which was variable from sub-millimeter to 6 mm, and Young’s modulus ∼1.6 ± 0.4 MPa. This new proof-of-concept fibers photofabrication approach opens lucrative opportunities for facile three-dimensional fabrication of hollow-core biostructures with controllable geometry.

【 授权许可】

Unknown