Generation and Application of Fluorescent Anti-Human β2-Microglobulin VHHs via Amino Modification

Molecules

Lingyun Jia¹  Da Li¹  Fangling Ji¹  Chundong Huang¹  Jun Ren¹ 

[1] Liaoning Key Laboratory of Molecular Recognition and Imaging, School of Bioengineering, Dalian University of Technology, No.2 Linggong Road, Dalian 116023, Liaoning, China;

关键词: VHHs;    single domain antibodies;    amino modification;    protein structures;    fluorescent;   

DOI  :  10.3390/molecules24142600

来源: DOAJ

【 摘 要 】

The functionalization of VHHs enables their application in almost every aspect of biomedical inquiry. Amino modification remains a common strategy for protein functionalization, though is considered to be inferior to site-specific methods and cause protein property changes. In this paper, four anti-β2M VHHs were selected and modified on the amino group by NHS-Fluo. The impacts of amino modification on these VHHs were drastically different, and among all th examples, the modified NB-1 maintained the original stability, bioactivity and homogeneity of unmodified NB-1. Specific recognition of VHHs targeting β2M detected by fluorescence imaging explored the possible applications of VHHs. Via this study, we successfully functionalized the anti-β2M VHHs through amino modification and the results are able to instruct the simple and fast functionalization of VHHs in biomedical researches.

【 授权许可】

Unknown