OPTIMIZATION OF Chlorella vulgaris BEIJ. CULTIVATION IN A BIOREACTOR OF CONTINUOUS ACTION

Biotechnologia Acta

Viniarska¹  Grubinko¹  Palchyk¹  Burega¹  Bodnar¹ 

[1] Volodymyr Gnatiuk Ternopil National Pedagogical University;

关键词: chlorella;    photobioreactor;    continuous cultivation;   

DOI  :  10.15407/biotech9.04.042

来源: DOAJ

【 摘 要 】

The aim of the research was the development and testing of bioreactor for intensive cultivation of algae Chlorella vulgaris Beij. with fully controlled conditions within the operating parameters according to the selected evaluation criteria of the cultivation process. To check the functioning efficiency of the decigned photobioreactor, the growth of Chlorella vulgaris Beij. (CHLOROPHYTA) in Fitzgerald’s medium modified by Zender and Gorham (No 11) under the artificial illumination with daylight electric lamps (intensity of 2 500 Lx) for 16 hours a day at 22–25 ºС was studed. It was found that at stabilization of culture conditions the maximum value of culture density was observed at the 18th day of cultivation. In this moment, the amount of cells reached 269.2 ± 3.0·109 cells/l, while cells amount in stationary phase was within 110.1 ± 4.9·109 cells/l. This makes it possible the continuous chlorella cultivation with an average productivity in stationary mode of about 110 ± 4 mg/l of dry mass with protein content about 60 mg, carbohydratesabout 35 and lipids about 12 mg of dry mass/l. Sunlight and activators of biosynthesis of organic substances of individual classes allow changeing the ratio of proteins, carbohydrates and lipids that is prospective for further research.

【 授权许可】

Unknown