Comparative phenolic content and antioxidant activity of some medicinal plant extracts prepared by choline chloride based green solvents and methanol

Current Research in Green and Sustainable Chemistry

Neha Sharma¹  Dhiraj Vyas²  Pooja Choudhary³  K.H. Salaria³  Villayat Ali³  Rashmi Chalotra³  Sanjay Guleria³ 

[1] Corresponding author.;Biodiversity Division, CSIR-Indian Institute of Integrative Medicine, Canal Road, Jammu, Jammu and Kashmir, 180001, India;Natural Product-cum-Nano Lab, Division of Biochemistry, Faculty of Basic Sciences, Sher-e- Kashmir University of Agricultural Sciences and Technology of Jammu, Main Campus Chatha, Jammu- 180009, Jammu and Kashmir, India;

关键词: NADESs;    HPLC;    Choline chloride;    Malic acid;    Antioxidant activity;    α-glucosidase inhibitory activity;   

DOI  :  

来源: DOAJ

【 摘 要 】

In the present study, three north-western Himalayan medicinal plants, Ocimum sanctum, Terminalia bellerica and Terminalia chebula were used to evaluate the effectiveness of choline chloride based natural deep eutectic solvents (NADESs) in extracting the polyphenolic compounds. The natural deep eutectic solvents used in the study were choline chloride:glucose: water, choline chloride:fructose:water, choline chloride: xylose: water, choline chloride:glycerol:water and choline chloride:malic acid:water.The results of the above study showed that all the eutectic solvents were more effective in extracting phenolic compounds than methanol, however, choline chloride:malic acid:water based NADES was most effective among all the NADESs tested and methanol. Additionally, profiling of phenolic compounds in extracts obtained by choline chloride:malic acid:water and methanol was also carried out using HPLC analysis. It was also observed that extracts possessing higher polyphenolic content showed higher DPPH radical scavenging activity, ferric reducing antioxidant power, reducing power and α-glucosidase inhibitory activity. Our results suggests that natural deep eutectic solvents offers efficient and safe alternative to methanol for extraction of phenolic compounds from medicinal plants.

【 授权许可】

Unknown