Genetic Relatedness and Diversity of Staphylococcus aureus from Different Reservoirs: Humans and Animals of Livestock, Poultry, Zoo, and Aquaculture

Microorganisms

NarcisaM. Bandarra¹  Lurdes Clemente²  Eugénia Ferreira³  Vera Manageiro³  Manuela Caniça³  Vanessa Salgueiro³ 

[1] Divisão de Aquacultura, Valorização e Bioprospeção, Departamento do Mar e Recursos Marinhos, Instituto Português do Mar e da Atmosfera, 1749-077 Lisboa, Portugal;INIAV–Instituto Nacional de Investigação Agrária e Veterinária, 2780-157 Oeiras, Portugal;National Reference Laboratory of Antibiotic Resistances and Healthcare Associated Infections (NRL-AMR-HAI), Department of Infectious Diseases, National Institute of Health Dr. Ricardo Jorge, 1649-016 Lisbon, Portugal;

关键词: MRSA;    ST398;    Portugal;    animals;    human isolates;   

DOI  :  10.3390/microorganisms8091345

来源: DOAJ

【 摘 要 】

The main aim of this study was the characterization of antibiotic resistance mechanisms in 82 Staphylococcus aureus strains isolated from humans and animals. Antibiotic susceptibility testing was performed on all S. aureus isolates accordingly, and antibiotic-resistant genes were investigated by genotypic methods. The genetic diversity of S. aureus was studied through spa, multilocus sequence typing (MLST), and agr typing methods. The majority of S. aureus from human sources were resistant to cefoxitin (and harbor the mecA gene) and fluoroquinolones, whereas only four strains of S. aureus from animal sources revealed resistance to ciprofloxacin. In the set of S. aureus isolated from humans, the most frequent spa, MLST, and agr group were t032, ST22, and I, respectively. In strains from animal origin the most common spa, MLST, and agr group found were t2383, ST398, and III/not typable, respectively. S. aureus from humans and animals were identified either in clonal complexes CC5, CC30, and CC398, suggesting that they have the same putative founder in their evolution. Considering the three CCs encompassing strains from human and animal reservoirs with different spa-types, we can hypothesize that this might reflect an adaptation to different phylogenetic lineages in those reservoirs (host species) probably associated to genetic diversification of pre-existing strains.

【 授权许可】

Unknown