Quantum Dot-Based Molecular Beacon to MonitorIntracellular MicroRNAs

Sensors

Javed Ahmed¹  Bahy A. Ali¹  Abdullah M. Al Salem¹  Daoud Ali²  Abdulaziz A. Al-Khedhairy²  Sung Ung Moon³  Yong Seung Lee³  Soonhag Kim³  Jonghwan Lee³ 

[1] Aljeraisy DNA Research Chair, Department of Zoology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia;Department of Zoology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia;Institute for Bio-Medical Convergence, College of Medicine, Kwandong Catholic University, Gangneung-si, Gangwon-do 270-701, Korea;

关键词: quantum dot;    microRNA;    molecular beacon;    neurogenesis;   

DOI  :  10.3390/s150612872

来源: DOAJ

【 摘 要 】

Fluorescence monitoring of endogenous microRNA (miRNA or miR) activity related to neuronal development using nano-sized materials provides crucial information on miRNA expression patterns in a noninvasive manner. In this study, we report a new method to monitor intracellular miRNA124a using quantum dot-based molecular beacon(R9-QD-miR124a beacon). The R9-QD-miR124a beacon was constructed using QDs and two probes, miR124a-targeting oligomer and arginine rich cell-penetrating peptide(R9 peptide). The miR124a-targeting oligomer contains a miR124a binging sequence and a black hole quencher 1 (BHQ1). In the absence of target miR124a, the R9-QD-miR124a beacon forms a partial duplex beacon and remained in quenched state because the BHQ1 quenches the fluorescence signal of the R9-QD-miR124a beacon. The binding of miR124a to the miR124a binding sequence of the miR124a-targeting oligomer triggered the separation of the BHQ1 quencher and subsequent signal-on of a red fluorescence signal. Moreover, enhanced cellular uptake was achieved by conjugation with the R9 peptide, which resulted in increased fluorescent signal of the R9-QD-miR124a beacons in P19 cells during neurogenesis due to the endogenous expression of miR124a.

【 授权许可】

Unknown