Laccase Immobilization on Poly(p-Phenylenediamine)/Fe3O4 Nanocomposite for Reactive Blue 19 Dye Removal

Applied Sciences

Mingyang Yan¹  Youxun Liu¹  Yuanyuan Geng¹  Juan Huang¹ 

[1] School of Basic Medical Sciences, Xinxiang Medical University, Jinsui Avenue 601, Xinxiang 453003, China;

关键词: laccase;    poly(p-phenylenediamine);    reactive blue 19;    magnetic nanoparticles;    removal;    dye;    wastewater treatment;   

DOI  :  10.3390/app6080232

来源: DOAJ

【 摘 要 】

Magnetic poly(p-phenylenediamine) (PpPD) nanocomposite was synthesized via mixing p-phenylenediamine solution and Fe3O4 nanoparticles and used as a carrier for immobilized enzymes. Successful synthesis of PpPD/Fe3O4 nanofiber was confirmed by transmission electron microscopy and Fourier transform infrared spectroscopy. Laccase (Lac) was immobilized on the surface of PpPD/Fe3O4 nanofiber through covalent bonding for reactive blue 19 dye removal. The immobilized Lac-nanofiber conjugates could be recovered from the reaction solution using a magnet. The optimum reaction pH and temperature for the immobilized Lac were 3.5 and 65°C, respectively. The storage, operational stability, and thermal stability of the immobilized Lac were higher than those of its free counterpart. The dye removal efficiency of immobilized Lac was about 80% in the first 1 h of incubation, while that of free Lac was about 20%. It was found that the unique electronic properties of PpPD might underlie the high dye removal efficiency of immobilized Lac. Over a period of repeated operation, the dye removal efficiency was above 90% during the first two cycles and remained at about 43% after eight cycles. Immobilized Lac on PpPD/Fe3O4 nanofiber showed high stability, easy recovery, reuse capabilities, and a high removal efficiency for reactive blue 19 dye; therefore, it provides an optional tool for dye removal from wastewater.

【 授权许可】

Unknown