Extraction and Separation of Eight Ginsenosides from Flower Buds of Panax Ginseng Using Aqueous Ionic Liquid-Based Ultrasonic-Assisted Extraction Coupled with an Aqueous Biphasic System

Molecules

Jinsong Zhang¹  Jianpeng Dou¹  Xingguang Su²  Qing Liang³  Qingwei Meng⁴ 

[1] College of Biological and Agricultural Engineering, Jilin University, Changchun 130022, China;College of Chemistry, Jilin University, Changchun 130012, China;College of Life Sciences, Jilin University, Changchun 130012, China;Plusone Native Co., Ltd., Changchun 130012, China;

关键词: response surface methodology;    ionic liquids;    ginsenosides;    ginseng flower buds;    ultrasonic-assisted extraction;    aqueous biphasic system;    separation;   

DOI  :  10.3390/molecules24040778

来源: DOAJ

【 摘 要 】

Ionic liquids (ILs) are recognized as a possible replacement of traditional organic solvents, and ILs have been widely applied to extract various compounds. The present work aims to extract ginsenosides from Panax ginseng flower buds using aqueous ionic liquid based ultrasonic assisted extraction (IL-UAE). The extraction yields of 1-alkyl-3-methylimidazolium ionic liquids with different anions and alkyl chains were evaluated. The extraction parameters of eight ginsenosides were optimized by utilizing response surface methodology (RSM). The model demonstrated that a high yield of total ginsenosides could be obtained using IL-UAE, and the optimum extraction parameters were 0.23 M [C₄mim][BF₄], ultrasonic time of 23 min, temperature of extraction set to 30 °C, and liquid-solid ratio of 31:1. After that, an aqueous biphasic system (ABS) was used to separate ginsenosides further. The nature and concentration of salt, as well as the value of pH in ionic liquid were evaluated, and the optimal conditions (6.0 mL IL extract, 3 g NaH₂PO₄, and pH 5.0) were obtained. The preconcentration factor was 2.58, and extraction efficiency reached 64.53%. The results indicate that as a simple and efficient method, an IL-UAE-ABS can be considered as a promising method for extracting and separating the natural active compounds from medicinal herbs.

【 授权许可】

Unknown