Differences in the Active Endometrial Microbiota across Body Weight and Cancer in Humans and Mice

Cancers

Rhonda Farrell¹  Neil Lambie²  Farokh Fargah³  Ellen M. Olzomer⁴  Kyle L. Hoehn⁴  Frances L. Byrne⁴  Nadeem O. Kaakoush⁵  Amy R. Martin⁶  Melidya Kosasih⁶  Daniella Susic⁶ 

[1] Chris O’Brien Lifehouse, Camperdown, NSW 2050, Australia;Department of Anatomical Pathology, Canterbury Health Laboratories, Christchurch 8011, New Zealand;Laverty Pathology-North Ryde, Macquarie Park, Sydney, NSW 2113, Australia;School of Biotechnology and Biomolecular Sciences, Faculty of Science, University of New South Wales, Sydney, NSW 2052, Australia;School of Medical Sciences, Faculty of Medicine, University of New South Wales, Sydney, NSW 2052, Australia;School of Women’s and Children’s Health, Faculty of Medicine, University of New South Wales, Sydney, NSW 2052, Australia;

关键词: uterine cancer;    microbiota;    lactobacillus;    obesity;   

DOI  :  10.3390/cancers14092141

来源: DOAJ

【 摘 要 】

Obesity is a risk factor for endometrial cancer. The aim of this study was to determine whether actively replicating microbiota in the endometrium differ between obese vs. lean and cancer vs. benign states. We performed 16S rRNA amplicon sequencing on endometrial tissues from lean and obese women with and without endometrial cancer, and lean and obese mice. Results displayed human endometrial microbiota clustered into three community types (R = 0.363, p = 0.001). Lactobacillus was dominant in community type 1 (C1) while community type 2 (C2) had high levels of Proteobacteria and more cancer samples when compared to C1 (p = 0.007) and C3 (p = 0.0002). A significant increase in the prevalence of the C2 community type was observed across body mass index and cancer (χ² = 14.24, p = 0.0002). The relative abundance of Lactobacillus was lower in cancer samples (p = 0.0043), and an OTU with 100% similarity to Lactobacillus iners was enriched in control samples (p = 0.0029). Mouse endometrial microbiota also clustered into three community types (R = 0.419, p = 0.001) which were not influenced by obesity. In conclusion, obesity and cancer are associated with community type prevalence in the human endometrium, and Lactobacillus abundance is associated with normal uterine histologies in humans and mice.

【 授权许可】

Unknown