CRISPR-Based Editing Techniques for Genetic Manipulation of Primary T Cells

Methods and Protocols

Mateusz Kotowski¹  Sumana Sharma¹ 

[1] MRC Human Immunology Unit, John Radcliffe Hospital, University of Oxford, Oxford OX3 9DS, UK;

关键词: primary T cells;    CRISPR/Cas9;    genome-editing;    CAR-T cells;   

DOI  :  10.3390/mps3040079

来源: DOAJ

【 摘 要 】

While clustered regularly interspaced short palindromic repeats (CRISPR)-based genome editing techniques have been widely adapted for use in immortalised immune cells, efficient manipulation of primary T cells has proved to be more challenging. Nonetheless, the rapid expansion of the CRISPR toolbox accompanied by the development of techniques for delivery of CRISPR components into primary T cells now affords the possibility to genetically manipulate primary T cells both with precision and at scale. Here, we review the key features of the techniques for primary T cell editing and discuss how the new generation of CRISPR-based tools may advance genetic engineering of these immune cells. This improved ability to genetically manipulate primary T cells will further enhance our fundamental understanding of cellular signalling and transcriptional networks in T cells and more importantly has the potential to revolutionise T cell-based therapies.

【 授权许可】

Unknown