Plasma Lipid Profiles Change with Increasing Numbers of Mild Traumatic Brain Injuries in Rats

Metabolites

Harrison Szemray¹  Nathan G. Lawler¹  Melissa Papini²  Chidozie C. Anyaegbu²  Carole Bartlett²  Terence McGonigle²  Brittney Lins²  Melinda Fitzgerald²  Sarah C. Hellewell²  Ryan S. Anderton³  Kerry Leggett³  Luke Whiley³ 

[1]Australian National Phenome Centre, Health Futures Institute, Murdoch University, Murdoch, WA 6150, Australia

[2]Curtin Health Innovation Research Institute, Curtin University, Bentley, WA 6102, Australia

[3]Perron Institute for Neurological and Translational Science, Ralph and Patricia Sarich Neuroscience Research Institute Building, Nedlands, WA 6009, Australia

关键词: lipidomics;    lipid;    mild traumatic brain injury;    repeated mild traumatic brain injury;    liquid chromatography–mass spectrometry;   

DOI  :  10.3390/metabo12040322

来源: DOAJ

【 摘 要 】

Mild traumatic brain injury (mTBI) causes structural, cellular and biochemical alterations which are difficult to detect in the brain and may persist chronically following single or repeated injury. Lipids are abundant in the brain and readily cross the blood-brain barrier, suggesting that lipidomic analysis of blood samples may provide valuable insight into the neuropathological state. This study used liquid chromatography-mass spectrometry (LC-MS) to examine plasma lipid concentrations at 11 days following sham (no injury), one (1×) or two (2×) mTBI in rats. Eighteen lipid species were identified that distinguished between sham, 1× and 2× mTBI. Three distinct patterns were found: (1) lipids that were altered significantly in concentration after either 1× or 2× F mTBI: cholesterol ester CE (14:0) (increased), phosphoserine PS (14:0/18:2) and hexosylceramide HCER (d18:0/26:0) (decreased), phosphoinositol PI(16:0/18:2) (increased with 1×, decreased with 2× mTBI); (2) lipids that were altered in response to 1× mTBI only: free fatty acid FFA (18:3 and 20:3) (increased); (3) lipids that were altered in response to 2× mTBI only: HCER (22:0), phosphoethanolamine PE (P-18:1/20:4 and P-18:0/20:1) (increased), lysophosphatidylethanolamine LPE (20:1), phosphocholine PC (20:0/22:4), PI (18:1/18:2 and 20:0/18:2) (decreased). These findings suggest that increasing numbers of mTBI induce a range of changes dependent upon the lipid species, which likely reflect a balance of damage and reparative responses.

【 授权许可】

Unknown