MAP3K4 Controls the Chromatin Modifier HDAC6 during Trophoblast Stem Cell Epithelial-to-Mesenchymal Transition

Cell Reports

Kayley Abell-Hart¹  Amy N. Abell¹  Ramin Homayouni¹  Robert J. Mobley¹  Deepthi Raghu¹  Lauren D. Duke¹  Sujoy Roy¹  Kyla Lutz²  Shawn M. Gomez²  Gary L. Johnson³  Jon S. Zawistowski³ 

[1] Department of Biological Sciences, University of Memphis, Memphis, TN 38152, USA;Department of Biomedical Engineering and Curriculum in Bioinformatics and Computational Biology, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA;Department of Pharmacology and Lineberger Comprehensive Cancer Center, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA;

关键词: epithelial to mesenchymal transition;    epigenetics;    chromatin remodelers;    MAP3K4;    trophoblast stem cells;    histone deacetylase;    HDAC6;    tight junctions;    histone acetylation;    development;   

DOI  :  10.1016/j.celrep.2017.02.030

来源: DOAJ

【 摘 要 】

The first epithelial-to-mesenchymal transition (EMT) occurs in trophoblast stem (TS) cells during implantation. Inactivation of the serine/threonine kinase MAP3K4 in TS cells (TSKI4 cells) induces an intermediate state of EMT, where cells retain stemness, lose epithelial markers, and gain mesenchymal characteristics. Investigation of relationships among MAP3K4 activity, stemness, and EMT in TS cells may reveal key regulators of EMT. Here, we show that MAP3K4 activity controls EMT through the ubiquitination and degradation of HDAC6. Loss of MAP3K4 activity in TSKI4 cells results in elevated HDAC6 expression and the deacetylation of cytoplasmic and nuclear targets. In the nucleus, HDAC6 deacetylates the promoters of tight junction genes, promoting the dissolution of tight junctions. Importantly, HDAC6 knockdown in TSKI4 cells restores epithelial features, including cell-cell adhesion and barrier formation. These data define a role for HDAC6 in regulating gene expression during transitions between epithelial and mesenchymal phenotypes.

【 授权许可】

Unknown