Highly thermal stable RNase A@PbS/ZnS quantum dots as NIR-IIb image contrast for visualizing temporal changes of microvasculature remodeling in flap

Journal of Nanobiotechnology

Hao Wang¹  Shixian Dong²  Amr M. Abdou³  Yuefeng Hao⁴  Xing Yang⁴  Pingkang Qian⁵  Liman Sai⁶  Shaoqing Feng⁷  Chen Chen⁸  Dongling Ma⁹  Peng Wang^1,10  Jun Chen^1,10  Yunxia Li^1,10  Yimeng Yang^1,10  Mo Chen^1,10  Shiyi Chen^1,10  Sijia Feng^1,10 

[1] Asia Cellular Therapeutics (Shanghai) Co., Ltd;Department of Anatomy and Physiology, School of Medicine, Shanghai Jiao Tong University;Department of Microbiology and Immunology, National Research Center;Department of Orthopedics, Affiliated Suzhou Hospital of Nanjing Medical University;Department of Orthopedics, Kunshan Hospital of Traditional Chinese Medicine;Department of Physics, Shanghai Normal University;Department of Plastic and Reconstructive Surgery, Shanghai Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University;Department of Sports Medicine, Shanghai Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University;Institut National de la Recherche Scientifique Centre Énergie Matériaux et Télécommunications, Université du Québec;Sports Medicine Institute of Fudan Univerisity, Department of Sports Medicine and Arthroscopy, Huashan Hospital, Fudan University;

关键词: Thermal stability;    Vascular imaging;    Quantum dots;    NIR-IIb window;    Intraoperative imaging navigation;   

DOI  :  10.1186/s12951-022-01312-0

来源: DOAJ

【 摘 要 】

Abstract Surgeons face great challenges in acquiring high-performance imaging because fluorescence probes with desired thermal stability remains rare. Here, hybrid lead sulfide/zinc sulfide quantum dots (PbS/ZnS QDs) nanostructures emitting in the long-wavelength end of the second near-infrared (NIR-IIb) window were synthesized and conjugated with Ribonuclease-A (RNase A). Such formed RNase A@PbS/ZnS QDs exhibited strong NIR IIb fluorescence and thermal stability, as supported by the photoluminescent emission assessment at different temperatures. This will allow the RNase A@PbS/ZnS QDs to provide stable fluorescence signals for long-time intraoperative imaging navigation, despite often happened, undesirable thermal accumulation in vivo. Compared to NIR-IIa fluorescence imaging, NIR-IIb vascular fluorescence imaging achieved larger penetration depth, higher signal/background ratios and nearly zero endogenous tissue autofluorescence. Moreover, these QDs illustrate the reliability during the real-time and long-time precise assessment of flap perfusion by clearly visualizing microvasculature map. These findings contribute to intraoperative imaging navigation with higher precision and lower risk. Graphical Abstract

【 授权许可】

Unknown