Foods | |
AGL9: A Novel Hepatoprotective Peptide from the Larvae of Edible Insects Alleviates Obesity-Induced Hepatic Inflammation by Regulating AMPK/Nrf2 Signaling | |
Byung-gyu Kim1  Young-Jin Choi2  Eun-Kyung Kim2  Bokyung Lee2  Meiqi Fan3  Sung Mun Bae4  Ji Hye Kim4  Yujiao Tang5  | |
[1] Center for Genomic Integrity, Institute for Basic Science, Ulsan 44919, Korea;Department of Food Science and Nutrition, College of Health Science, Dong-A University, Busan 49315, Korea;Division of Food Bioscience, College of Biomedical and Health Sciences, Konkuk University, Chungju 27478, Korea;Gyeongnam Agricultural Research and Extension Services, Jinju 52733, Korea;School of Life Sciences, Changchun University of Science and Technology, Changchun 130600, China; | |
关键词: hepatoprotective peptide; AGL9; hepatic lipid metabolism; inflammation; nonalcoholic fatty liver disease; | |
DOI : 10.3390/foods10091973 | |
来源: DOAJ |
【 摘 要 】
In this study, we investigated the anti-obesity properties of the novel peptide Ala-Gly-Leu-Gln-Phe-Pro-Val-Gly-Arg (AGL9), isolated from the enzymatic hydrolysate of Allomyrinadichotoma larvae. To investigate the preventive effects of AGL9 against hepatic steatosis and its possible mechanisms of action, we established an nonalcoholic fatty liver disease (NAFLD) model by feeding C57BL/6 mice a high-fat diet. NAFLD mice were administered 100 mg/kg AGL9 and 60 mg/kg orlistat via gavage (10 mL/kg) for 5 weeks, followed by the collection of blood and liver tissues. We found that AGL9 normalized the levels of serum alanine aminotransferase, aspartate aminotransferase, triglyceride, total cholesterol, high-density lipoprotein, very low-density lipoprotein (LDL)/LDL, adiponectin, and leptin in these mice. Additionally, AGL9 activated the protein-level expression of 5′ AMP-activated protein kinase and acetyl-CoA carboxylase phosphorylation and the transcript-level expression of sterol regulatory element-binding protein-1c, fatty acid synthase, superoxide dismutase, glutathione peroxidase, glucocorticoid receptor, nuclear respiratory factor 2, tumor necrosis factor-α, interleukin-1β, interleukin-6, and monocyte chemoattractant protein-1 in hepatocytes. These results showed that AGL9 exhibited hepatoprotective effects by attenuating lipid deposition, oxidative stress, and inflammation via inhibition of AMPK/Nrf2 signaling, thereby reducing the production of hepatic proinflammatory mediators and indicating AGL9 as a potential therapeutic strategy for NAFLD.
【 授权许可】
Unknown