Intracellular phase for an extracellular bacterial pathogen:MgtC shows the way

Microbial Cell

Chantal Soscia¹  Sophie Bleves¹  Claudine Belon²  Audrey Bernut²  Anne-Béatrice Blanc-Potard² 

[1] CNRS and Aix-Marseille University, Laboratoire d’Ingénierie des Systèmes Macromoléculaires (UMR7255), IMM, 31 Chemin Joseph Aiguier, 13402 Marseille cedex 20, France.;Laboratoire de Dynamique des Interactions Membranaires Normales et Pathologiques, Université de Montpellier (DIMNP CNRS-UMR5235), Place Eugène Bataillon, 34095 Montpellier Cedex 05, France.;

关键词: Pseudomonas aeruginosa;    MgtC;    macrophage;    magnesium;    biofilm;    zebrafish;   

DOI  :  10.15698/mic2015.09.227

来源: DOAJ

【 摘 要 】

Pseudomonas aeruginosa is an extracellular pathogen known to impair host phagocytic functions. However, our recent results identify MgtC as a novel actor in P. aeruginosa virulence,whichplaysaroleinanintramacrophagephaseofthispathogen.Inagreement with its intracellular function, P. aeruginosa mgtC gene expressionisstronglyinducedwhenthebacteriareside within macrophages. MgtC was previously known asahorizontally-acquiredvirulencefactorimportant for multiplication inside macrophages in several intracellular bacterial pathogens. MgtC thus provides a singular example of a virulence determinant that subverts macrophagesbothinintracellularandextracellular pathogens.Moreover,wedemonstratethat P.aeru-ginosa MgtCisrequiredforoptimalgrowthinMg2+ deprived medium, a property shared by MgtC factors fromintracellularpathogensand,underMg2+ limitation, P.aeruginosaMgtCpreventsbiofilmformation. We propose that MgtC has a similar function in intracellular and extracellular pathogens, which contributes to macrophage resistance and fine-tune adaptation to the host in relation to the different bacterial lifestyles. MgtC thus appears as an attractive target for antivirulence strategies and our work provides a natural peptide as MgtC antagonist, which paves the way for the development of MgtC inhibitors.

【 授权许可】

Unknown