Rice Senescence-Induced Receptor-Like Kinase (OsSRLK) Is Involved in Phytohormone-Mediated Chlorophyll Degradation

International Journal of Molecular Sciences

DoThi Trang¹  Soo-Cheul Yoo¹  Jadamba Chuluuntsetseg¹  Joon-Kwan Moon¹  Kiyoon Kang²  Yo-Han Yoo³  Woo-Jong Hong³  Ki-Hong Jung³  Na-Hyun Shin⁴ 

[1] Department of Plant Life and Environmental Science, Hankyong National University, 327, Jungangro, Anseong-si, Gyeonggi-do 17579, Korea;Department of Plant Science, Plant Genomics and Breeding Institute, Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Korea;Graduate School of Biotechnology and Crop Biotech Institute, Kyung Hee University, Yongin 446-701, Korea;Graduate School of Integrated Bioindustry, Sejong University, Seoul 05006, Korea;

关键词: rice;    receptor-like kinase;    rna sequencing;    chlorophyll degradation;    leaf senescence;    phytohormone;   

DOI  :  10.3390/ijms21010260

来源: DOAJ

【 摘 要 】

Chlorophyll breakdown is a vital catabolic process of leaf senescence as it allows the recycling of nitrogen and other nutrients. In the present study, we isolated rice senescence-induced receptor-like kinase (OsSRLK), whose transcription was upregulated in senescing rice leaves. The detached leaves of ossrlk mutant (ossrlk) contained more green pigment than those of the wild type (WT) during dark-induced senescence (DIS). HPLC and immunoblot assay revealed that degradation of chlorophyll and photosystem II proteins was repressed in ossrlk during DIS. Furthermore, ultrastructural analysis revealed that ossrlk leaves maintained the chloroplast structure with intact grana stacks during dark incubation; however, the retained green color and preserved chloroplast structures of ossrlk did not enhance the photosynthetic competence during age-dependent senescence in autumn. In ossrlk, the panicles per plant was increased and the spikelets per panicle were reduced, resulting in similar grain productivity between WT and ossrlk. By transcriptome analysis using RNA sequencing, genes related to phytohormone, senescence, and chlorophyll biogenesis were significantly altered in ossrlk compared to those in WT during DIS. Collectively, our findings indicate that OsSRLK may degrade chlorophyll by participating in a phytohormone-mediated pathway.

【 授权许可】

Unknown