Antibiotic Resistance of Escherichia coli Isolated from Conventional, No Antibiotics, and Humane Family Owned Retail Broiler Chicken Meat

Animals

JenniferA. Jay¹  Amelia Reynolds¹  Mckenzie Duran-Gonzalez¹  Alicia Amundson¹  Vanessa Thulsiraj¹  HelenM. Sanchez¹  VictoriaA. Whitener¹  Carolyn Collins²  Benjamin Schmidt²  Andrea Maben²  Edwin Giragossian²  Elizabeth Roswell²  Allison Hornstra²  Lauren Sevigny²  Cindy Xiong²  Sarah Kamel² 

[1] Department of Civil and Environmental Engineering, University of California at Los Angeles, Los Angeles, CA 90095, USA;Institute of the Environment and Sustainability, University of California at Los Angeles, Los Angeles, CA 90095, USA;

关键词: antibiotic resistance;    antimicrobial resistance;    poultry;    chicken;    ampicillin;    erythromycin;   

DOI  :  10.3390/ani10122217

来源: DOAJ

【 摘 要 】

The use of antibiotics for therapeutic and especially non-therapeutic purposes in livestock farms promotes the development of antibiotic resistance in previously susceptible bacteria through selective pressure. In this work, we examined E. coli isolates using the standard Kirby-Bauer disk diffusion susceptibility protocol and the CLSI standards. Companies selling retail chicken products in Los Angeles, California were grouped into three production groupings—Conventional, No Antibiotics, and Humane Family Owned. Humane Family Owned is not a federally regulated category in the United States, but shows the reader that the chicken is incubated, hatched, raised, slaughtered, and packaged by one party, ensuring that the use of antibiotics in the entire production of the chicken is known and understood. We then examined the antibiotic resistance of the E. coli isolates (n = 325) by exposing them to seven common antibiotics, and resistance was seen to two of the antibiotics, ampicillin and erythromycin. As has been shown previously, it was found that for both ampicillin and erythromycin, there was no significant difference (p > 0.05) between Conventional and USDA (United States Department of Agriculture)-certified No Antibiotics chicken. Unique to this work, we additionally found that Humane Family Owned chicken had fewer (p ≤ 0.05) antibiotic-resistant E. coli isolates than both of the previous. Although not considered directly clinically relevant, we chose to test erythromycin because of its ecological significance to the environmental antibiotic resistome, which is not generally done. To our knowledge, Humane Family Owned consumer chicken has not previously been studied for its antibiotic resistance. This work contributes to a better understanding of a potential strategy of chicken production for the overall benefit of human health, giving evidentiary support to the One Health approach implemented by the World Health Organization.

【 授权许可】

Unknown