Electron microscopy for ultrastructural analysis and protein localization in Saccharomyces cerevisiae

Microbial Cell

Muriel Mari¹  Andri Frankl¹  Fulvio Reggiori¹ 

[1] Department of Cell Biology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands.;

关键词: electron microscopy;    electron tomography;    immunolabeling;    chemical fixation;    cryo-immobilization;    correlative light and electron microscopy;    Saccharomyces cerevisiae;   

DOI  :  10.15698/mic2015.11.237

来源: DOAJ

【 摘 要 】

Theyeast Saccharomycescerevisiae isakeymodelsystemfor studyingofamultitudeofcellularprocessesbecauseofitsamenabilityto genetics,molecularbiologyandbiochemicalprocedures.Ultrastructuralexaminations of this organism, though, are traditionally difficult because of the presenceofathickcellwallandthehighdensity ofcytoplasmicproteins.A seriesofrecentmethodologicalandtechnicaldevelopments,however,has revivedinterestinmorphologicalanalysesofyeast (e.g.[1-3]).Herewepresentareviewofestablishedandnewmethods,from samplepreparationto imaging,fortheultrastructuralanalysisof S.cerevisiae.Weincludeinformation for the use of different fixation methods, embedding procedures, approachesforcontrastenhancement,andsamplevisualizationtechniques, withreferencestosuccessfulexamples.Thegoalof thisreviewistoguide researchers that want to investigate a particular process at the ultrastructural levelin yeast by aiding in the selection of the most appropriate approach to visualize a specific structure or subcellular compartment.

【 授权许可】

Unknown