Fibroblast Interaction with Different Abutment Surfaces: In Vitro Study

International Journal of Molecular Sciences

Stefano Carossa¹  Federico Mussano¹  Esperanza Gross Trujillo²  Guillermo Pradies²  Tullio Genova³  Sara Petrillo⁴  Maurizio Muzzi⁵  Luigi Canullo⁶ 

[1] CIR Dental School—Department of Surgical Sciences, University of Torino, Via Nizza 230, 10126 Turin, Italy;Department of Buccofacial Prosthesis, University Complutense, 28040 Madrid, Spain;Department of Life Sciences and Systems Biology, University of Torino, 10126 Turin, Italy;Department of Molecular Biotechnology and Health Sciences, University of Rome III, 00133 Rome, Italy;Department of Science, University of Rome III, 00133 Rome, Italy;Private Practice, Via Nizza, 46, 00198 Rome, Italy;

关键词: abutment integration;    abutment characteristics;    dental implant abutment;    in vitro study;    fibroblast;    bioactivation;    plasma of argon;    uv light;   

DOI  :  10.3390/ijms21061919

来源: DOAJ

【 摘 要 】

Background: Attaining an effective mucosal attachment to the transmucosal part of the implant could protect the peri-implant bone. Aim: To evaluate if chair side surface treatments (plasma of Argon and ultraviolet light) may affect fibroblast adhesion on different titanium surfaces designed for soft tissue healing. Methods: Grade 5 titanium discs with four different surface topographies were subdivided into 3 groups: argon-plasma; ultraviolet light, and no treatment. Cell morphology and adhesion tests were performed at 20 min, 24 h, and 72 h. Results: Qualitative observation of the surfaces performed at the SEM was in accordance with the anticipated features. Roughness values ranged from smooth (MAC Sa = 0.2) to very rough (XA Sa = 21). At 20 min, all the untreated surfaces presented hemispherical cells with reduced filopodia, while the cells on treated samples were more spread with broad lamellipodia. However, these differences in spreading behavior disappeared at 24 h and 72 h. Argon-plasma, but not UV, significantly increased the number of fibroblasts independently of the surface type but only at 20 min. Statistically, there was no surface in combination with a treatment that favored a greater cellular adhesion. Conclusions: Data showed potential biological benefits of treating implant abutment surfaces with the plasma of argon in relation to early-stage cell adhesion.

【 授权许可】

Unknown