Characterization of the Resistance to Powdery Mildew and Leaf Rust Carried by the Bread Wheat Cultivar Victo

International Journal of Molecular Sciences

Francesca Desiderio¹  Luigi Cattivelli¹  Elisabetta Mazzucotelli¹  Salim Bourras²  Beat Keller²  Giampiero Valè³  Diego Rubiales⁴ 

[1] CREA Research Centre for Genomics and Bioinformatics, 29017 Fiorenzuola d’Arda, Italy;Department of Plant and Microbial Biology, University of Zurich, 8008 Zurich, Switzerland;DiSIT-Dipartimento di Scienze e Innovazione Tecnologica, Università del Piemonte Orientale, 13100 Vercelli, Italy;Institute for Sustainable Agriculture, CSIC, 14004 Córdoba, Spain;

关键词: Triticum aestivum;    powdery mildew;    leaf rust;    molecular marker;    QTL;   

DOI  :  10.3390/ijms22063109

来源: DOAJ

【 摘 要 】

Leaf rust and powdery mildew are two important foliar diseases in wheat. A recombinant inbred line (RIL) population, obtained by crossing two bread wheat cultivars (‘Victo’ and ‘Spada’), was evaluated for resistance to the two pathogens at seedling stage. Upon developing a genetic map of 8726 SNP loci, linkage analysis identified three resistance Quantitative Trait Loci (QTLs), with ‘Victo’ contributing the resistant alleles to all loci. One major QTL (QPm.gb-7A) was detected in response to Blumeria graminis on chromosome 7A, which explained 90% of phenotypic variation (PV). The co-positional relationship with known powdery mildew (Pm) resistance loci suggested that a new source of resistance was identified in T. aestivum. Two QTLs were detected in response to Puccinia triticina: a major gene on chromosome 5D (QLr.gb-5D), explaining a total PV of about 59%, and a minor QTL on chromosome 2B (QLr.gb-2B). A positional relationship was observed between the QLr.gb-5D with the known Lr1 gene, but polymorphisms were found between the cloned Lr1 and the corresponding ‘Victo’ allele, suggesting that QLr.gb-5D could represent a new functional Lr1 allele. Lastly, upon anchoring the QTL on the T. aestivum reference genome, candidate genes were hypothesized on the basis of gene annotation and in silico gene expression analysis.

【 授权许可】

Unknown