An optimized retroviral toolbox for overexpression and genetic perturbation of primary lymphocytes

Biology Open

Jeroen W. J. van Heijst¹  Lieve E. H. van der Donk¹  Jet van der Spek¹  Louis S. Ates¹  Teunis B. H. Geijtenbeek¹  Tom van Duivenvoorde¹  Marieke S. ten Brink²  Coenraad P. Kuijl³ 

[1]Department of Experimental Immunology, Amsterdam institute for Infection and Immunity, Amsterdam UMC, University of Amsterdam, Meibergdreef 9, Amsterdam 1105 AZ, the Netherlands

[2]Division of Infectious Diseases and Center of Experimental and Molecular Medicine, Amsterdam University Medical Centers, University of Amsterdam, Amsterdam 1105 AZ, the Netherlands

[3]Medical Microbiology and Infection Control, Cancer Center Amsterdam, Amsterdam UMC, Vrije Universiteit Amsterdam, De Boelelaan 1117, Amsterdam 1081 HV, the Netherlands

关键词: retrovirus;    t cell;    lymphocytes;    overexpression;   

DOI  :  10.1242/bio.059032

来源: DOAJ

【 摘 要 】

Genetic manipulation of primary lymphocytes is crucial for both clinical purposes and fundamental research. Despite their broad use, we encountered a paucity of data on systematic comparison and optimization of retroviral vectors, the workhorses of genetic modification of primary lymphocytes. Here, we report the construction and validation of a versatile range of retroviral expression vectors. These vectors can be used for the knockdown or overexpression of genes of interest in primary human and murine lymphocytes, in combination with a wide choice of selection and reporter strategies. By streamlining the vector backbone and insert design, these publicly available vectors allow easy interchangeability of the independent building blocks, such as different promoters, fluorescent proteins, surface markers and antibiotic resistance cassettes. We validated these vectors and tested the optimal promoters for in vitro and in vivo overexpression and knockdown of the murine T cell antigen receptor. By publicly sharing these vectors and the data on their optimization, we aim to facilitate genetic modification of primary lymphocytes for researchers entering this field.

【 授权许可】

Unknown