Clinical Features and Molecular Characteristics of Methicillin-Susceptible Staphylococcus aureus Ocular Infection in Taiwan

Antibiotics

Yhu-Chering Huang¹  Lung-Kun Yeh²  David H. K. Ma²  Eugene Yu-Chuan Kang²  Hsin-Yuan Tan²  Hung-Chi Chen²  Kuo-Hsuan Hung²  Ching-Hsi Hsiao²  Yueh-Ling Chen² 

[1] College of Medicine, Chang Gung University, Taoyuan 333, Taiwan;Department of Ophthalmology, Chang Gung Memorial Hospital, Taoyuan 333, Taiwan;

关键词: Staphyloccus aureus;    MSSA;    ocular infection;    pulsed-field gel electrophoresis;    multilocus sequence typing;    Panton-Valentine leukocidin;   

DOI  :  10.3390/antibiotics10121445

来源: DOAJ

【 摘 要 】

This study analyzed the clinical features and molecular characteristics of methicillin-susceptible Staphylococcus aureus (MSSA) ocular infections in Taiwan and compared them between community-associated (CA) and health-care-associated (HA) infections. We collected S. aureus ocular isolates from patients at Chang Gung Memorial Hospital between 2010 and 2017. The infections were classified as CA or HA using epidemiological criteria, and the isolates were molecularly characterized using pulsed-field gel electrophoresis, multilocus sequence typing, and Panton-Valentine leukocidin (PVL) gene detection. Antibiotic susceptibility was evaluated using disk diffusion and an E test. A total of 104 MSSA ocular isolates were identified; 46 (44.2%) were CA-MSSA and 58 (55.8%) were HA-MSSA. Compared with HA-MSSA strains, CA-MSSA strains caused a significantly higher rate of keratitis, but a lower rate of conjunctivitis. We identified 14 pulsotypes. ST 7/pulsotype BA was frequently identified in both CA-MSSA (28.3%) and HA-MSSA (37.9%) cases. PVL genes were identified in seven isolates (6.7%). Both CA-MSSA and HA-MSSA isolates were highly susceptible to vancomycin, teicoplanin, tigecycline, sulfamethoxazole–trimethoprim, and fluoroquinolones. The most common ocular manifestations were keratitis and conjunctivitis for CA-MSSA and HA-MSSA, respectively. The MSSA ocular isolates had diverse molecular characteristics; no specific genotype differentiated CA-MSSA from HA-MSSA. Both strains exhibited similar antibiotic susceptibility.

【 授权许可】

Unknown