Genome-Wide Role of HSF1 in Transcriptional Regulation of Desiccation Tolerance in the Anhydrobiotic Cell Line, Pv11

International Journal of Molecular Sciences

TakahiroG. Yamada¹  Yusuke Hiki¹  Akira Funahashi¹  Shoko Tokumoto²  Takahiro Kikawada²  Richard Cornette³  Yugo Miyata³  Ruslan Deviatiiarov⁴  Elena Shagimardanova⁴  Olga Kozlova⁴  Oleg Gusev⁴  Yuki Yoshida⁵ 

[1] Department of Biosciences and Informatics, Keio University, Yokohama 223-8522, Japan;Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa 277-8562, Japan;Division of Biotechnology, Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO), Tsukuba 305-0851, Japan;Extreme Biology laboratory, Institute of Fundamental Medicine and Biology, Kazan Federal University, 420008 Kazan, Russia;Institute for Advanced Biosciences, Keio University, Tsuruoka 997-0017, Japan;

关键词: CRISPR/Cas9;    knockout;    rescue;    anhydrobiosis;    Polypedilum vanderplanki;    insect cell;   

DOI  :  10.3390/ijms22115798

来源: DOAJ

【 摘 要 】

The Pv11, an insect cell line established from the midge Polypedilum vanderplanki, is capable of extreme hypometabolic desiccation tolerance, so-called anhydrobiosis. We previously discovered that heat shock factor 1 (HSF1) contributes to the acquisition of desiccation tolerance by Pv11 cells, but the mechanistic details have yet to be elucidated. Here, by analyzing the gene expression profiles of newly established HSF1-knockout and -rescue cell lines, we show that HSF1 has a genome-wide effect on gene regulation in Pv11. The HSF1-knockout cells exhibit a reduced desiccation survival rate, but this is completely restored in HSF1-rescue cells. By comparing mRNA profiles of the two cell lines, we reveal that HSF1 induces anhydrobiosis-related genes, especially genes encoding late embryogenesis abundant proteins and thioredoxins, but represses a group of genes involved in basal cellular processes, thus promoting an extreme hypometabolism state in the cell. In addition, HSF1 binding motifs are enriched in the promoters of anhydrobiosis-related genes and we demonstrate binding of HSF1 to these promoters by ChIP-qPCR. Thus, HSF1 directly regulates the transcription of anhydrobiosis-related genes and consequently plays a pivotal role in the induction of anhydrobiotic ability in Pv11 cells.

【 授权许可】

Unknown