In vitro propagation of bastard balm (Melittis melissophyllum L.)

Herba Polonica

Szymborska-Sandhu Izabela¹  Węglarz Zenon¹  Pawełczak Anna¹  Bączek Katarzyna¹ 

[1] Laboratory of New Herbal Products, Department of Vegetable and Medicinal Plants, Warsaw University of Life Sciences – SGGW, Nowoursynowska 166 02-787 Warsaw, Poland;

关键词: in vitro;    axillary shoots;    ms/b5 medium;    naa;    ba;    iba;    ex vitro adaptation;    in vitro;    pędy boczne;    pożywka ms/b5;    naa;    ba;    iba;    adaptacja ex vitro;   

DOI  :  10.1515/hepo-2015-0023

来源: DOAJ

【 摘 要 】

An efficient method for in vitro propagation of bastard balm by enhanced axillary shoot branching has been developed. The material to establish in vitro culture was shoot tips collected from three-year-old plants in May. The shoots obtained from initial explants were placed on MS/B5 medium containing 0.1, 0.5 or 1.0 mg/l BA with 0.01 mg/l NAA or without the auxin. The highest number of shoots per explant was obtained on the medium with 1.0 mg/l BA (3.9 shoots per explant). For the rooting of shoots ½ MS/B5 with IBA (0.25, 0.50 and 1.0 mg/l) medium was used. The medium without plant growth regulators served as a control. The best root regeneration was observed on the medium without IBA (87.1% of cuttings rooted). IBA used in the medium for shoot rooting affected the morphological traits of obtained microcuttings but not affected their weight. Irrespective of auxin concentration in this medium, obtained microcuttings acclimated in ex vitro conditions very well.

【 授权许可】

Unknown