Analysis of bioactive constituents from the leaves of Amorpha fruticosa L.

Journal of Food and Drug Analysis

Sen Guo¹  Zhongxiao Ma¹  Xueqin Cui¹  Qingchao Liu¹  Naisheng Bai¹  Jing Guo¹  Li Zhang¹  Chi-Tang Ho²  Min-Hsiung Pan³  Ching-Shu Lai³ 

[1] College of Chemical Engineering, Department of Pharmaceutical Engineering, Northwest University, Xi'an, Shaanxi, China;Department of Food Science, Rutgers University, New Brunswick, NJ, USA;Institute of Food Science and Technology, National Taiwan University, Taipei, Taiwan;

关键词: Amorpha fruticosa L.;    cytotoxicity;    Leguminosae;    phenolics;    quantitative analysis;   

DOI  :  10.1016/j.jfda.2016.10.006

来源: DOAJ

【 摘 要 】

Amorpha fruticosa L. is a Chinese folk medicine and rich in polyphenols. Fifteen known compounds were isolated and identified from the leaves of A. fruticosa L. They are tephrosin (1), 6a,12a-dehydrodeguelin (2), vitexin (3), afrormosin-7-O-β-d-glucopyranoside (4), 2″-O-α-l-rhamnopyranosyl isovitexin (5), rutin (6), chrysoeriol (7), 7-O-methylluteolin (8), trans-p-coumaric acid (9), 2-benzyl-4,6-dihydroxybenzoic acid-4-O-β-d-glucopyranoside (10), formononetin (11), quercetin (12), apigenin (13), β-sitosterol (14), and β-daucosterol (15). Compounds 3, 4, 5, and 7–9 were isolated from A. fruticosa L. for the first time. Cytotoxicity of individual compounds 3–10 and 90% ethanol extract against human cancer cell lines HCT116 and HepG2 were reported. The results suggested that compounds 7 and 8, and the crude extract exhibited inhibitory effects on human cancer cell line HCT116, at concentrations of 100 μg/mL, 5 μg/mL, and 25 μg/mL at <60% of cell viability rate, respectively. In addition, a valid high-performance liquid chromatography diode array detector method was established to quantitatively analyze compounds 1–12 in the leaves of A. fruticosa L., which was harvested at three different stages of maturity from May 20 to August 10, 2014. The results demonstrated that contents were greatly influenced by the maturity. Total amounts of the analytical constituents gradually increased from May 20 to August 10, with the values ranging from 10.86 mg/g to 18.84 mg/g, whereas bioactive compounds 7 and 8 presented the opposite variation trend. The results of this study may provide data for further study and comprehensive utilization of A. fruticosa L. resource.

【 授权许可】

Unknown