Introgression of QTLs for Drought Tolerance into Farmers’ Preferred Sorghum Varieties

Agriculture

Rajaguru Bohar¹  Milcah Kigoni²  Ana Luísa Garcia-Oliveira³  Tileye Feyissa⁴  Santosh Deshpande⁵  Andekelile Mwamahonje⁶  Pangirayi Tongoona⁶  Kwadwo Ofori⁶  John Saviour Yaw Eleblu⁶ 

[1] CGIAR Excellence in Breeding Platform (EiB), CIMMYT, ICRISAT Campus, Hyderabad 502324, India;Department of Crop Science, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA;Excellence in Breeding (EiB) Platform, International Maize and Wheat Improvement Centre (CIMMYT), ICRAF, Nairobi 00621, Kenya;Institute of Biotechnology, Addis Ababa University, Addis Ababa P.O. Box 1176, Ethiopia;International Crops Research Institute for the Semi-Arid Tropics, Patancheru 502324, India;West Africa Centre for Crop Improvement (WACCI), College of Basic and Applied Sciences, University of Ghana, Accra 00233, Ghana;

关键词: STG;    post-flowering drought tolerance;    genotyping;    single nucleotide polymorphism;    marker-assisted selection;   

DOI  :  10.3390/agriculture11090883

来源: DOAJ

【 摘 要 】

Sorghum is a major staple food crop for the people in semi-arid areas of Africa and Asia. Post-flowering drought is a global constraint of sorghum production. The study aimed to improve stay-green (STG) characteristics of farmer-preferred sorghum varieties in Tanzania using marker-assisted backcrossing. A total of 752 individuals representing five BC₂F₁ populations and their parents were genotyped using previously reported KASP markers linked with STG 3A and STG 3B quantitative trait loci (QTL). In the BC₂F₁ populations, the maximum number of individuals with heterozygous alleles were observed in S35*Pato background (37) whereas only seven individuals derived from the B35*Wahi parents’ background contained heterozygous alleles. Of the 30 single nucleotide polymorphism (SNP) markers, favourable alleles were observed at 18 loci in BC₂F₁ populations. In the BC₂F₁ generation, the highest (0.127 kg/panicle) grain yield was observed in the B35*NACO Mtama 1 background population. The genotypic analysis revealed the presence of favourable alleles in homozygous conditions at markers loci associated with STG 3A and STG 3B QTLs in BC₂F₃ populations, suggesting successful introgression of STG QTLs from the donor parents to the recurrent parents. Across water irrigation regimes, the highest (0.068 kg/panicle) mean grain weight was observed in the genotype NA316C. Therefore, our study demonstrated the utility of marker-assisted backcrossing for drought tolerance improvement of locally adapted sorghum varieties in Africa.

【 授权许可】

Unknown