Agriculture | |
Introgression of QTLs for Drought Tolerance into Farmers’ Preferred Sorghum Varieties | |
Rajaguru Bohar1  Milcah Kigoni2  Ana Luísa Garcia-Oliveira3  Tileye Feyissa4  Santosh Deshpande5  Andekelile Mwamahonje6  Pangirayi Tongoona6  Kwadwo Ofori6  John Saviour Yaw Eleblu6  | |
[1] CGIAR Excellence in Breeding Platform (EiB), CIMMYT, ICRISAT Campus, Hyderabad 502324, India;Department of Crop Science, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA;Excellence in Breeding (EiB) Platform, International Maize and Wheat Improvement Centre (CIMMYT), ICRAF, Nairobi 00621, Kenya;Institute of Biotechnology, Addis Ababa University, Addis Ababa P.O. Box 1176, Ethiopia;International Crops Research Institute for the Semi-Arid Tropics, Patancheru 502324, India;West Africa Centre for Crop Improvement (WACCI), College of Basic and Applied Sciences, University of Ghana, Accra 00233, Ghana; | |
关键词: STG; post-flowering drought tolerance; genotyping; single nucleotide polymorphism; marker-assisted selection; | |
DOI : 10.3390/agriculture11090883 | |
来源: DOAJ |
【 摘 要 】
Sorghum is a major staple food crop for the people in semi-arid areas of Africa and Asia. Post-flowering drought is a global constraint of sorghum production. The study aimed to improve stay-green (STG) characteristics of farmer-preferred sorghum varieties in Tanzania using marker-assisted backcrossing. A total of 752 individuals representing five BC2F1 populations and their parents were genotyped using previously reported KASP markers linked with STG 3A and STG 3B quantitative trait loci (QTL). In the BC2F1 populations, the maximum number of individuals with heterozygous alleles were observed in S35*Pato background (37) whereas only seven individuals derived from the B35*Wahi parents’ background contained heterozygous alleles. Of the 30 single nucleotide polymorphism (SNP) markers, favourable alleles were observed at 18 loci in BC2F1 populations. In the BC2F1 generation, the highest (0.127 kg/panicle) grain yield was observed in the B35*NACO Mtama 1 background population. The genotypic analysis revealed the presence of favourable alleles in homozygous conditions at markers loci associated with STG 3A and STG 3B QTLs in BC2F3 populations, suggesting successful introgression of STG QTLs from the donor parents to the recurrent parents. Across water irrigation regimes, the highest (0.068 kg/panicle) mean grain weight was observed in the genotype NA316C. Therefore, our study demonstrated the utility of marker-assisted backcrossing for drought tolerance improvement of locally adapted sorghum varieties in Africa.
【 授权许可】
Unknown