期刊论文详细信息
Journal of Lipid Research
Modulation of paraoxonase 1 and 3 expression after moderate exercise training in the rat
Gianna Evelina De Medio1  Rosa Lapalombella1  Arsenio Veicsteinas2  Rita Romani2  Irene Pirisinu3  Vittoria Margonato3  Simona di Tullio4  Marina Marini5  Gabriella Rosi5 
[1] Motor and Sport Activity Laboratory, University of Perugia, Via Bambagioni, 06126 Perugia, Italy;Department of Experimental Medicine, Division of Cellular and Molecular Biology, Division of Biochemistry, University of Perugia, Via del Giochetto, 06122 Perugia, Italy;Department of Histology, Embryology, and Applied Biology, University of Bologna, Via Belmeloro, 8, 40126 Bologna, Italy;Division of Cellular and Molecular Biology, and Department of Internal Medicine, Division of Biochemistry, University of Perugia, Via del Giochetto, 06122 Perugia, Italy;Institute of Physical Exercise, Health and Sport Activities, University of Milan, Milan, Italy;
关键词: arylesterase;    carboxylesterase;    lactonase;    membrane phospholipids;    mild exercise training;    protein expression;   
DOI  :  
来源: DOAJ
【 摘 要 】

Paraoxonases (PONs) are a small family of antioxidant enzymes whose antiatherogenic activity is well known. The aim of the present study was the evaluation of the effects of moderate aerobic training on their expression using a rat model. In order to discriminate between PON1 and PON3 enzymatic activity, we took advantage of some differences in their substrate preferences. PON1 and PON3 enzymatic activities and their protein levels were analyzed in plasma and in liver microsomes, and their mRNA levels in the liver. Exercise training did not affect PON1 expression or enzymatic activity but increased PON3 mRNA, protein levels, and enzymatic activity. Training also induced variations in plasma membrane composition, including an increase in polyunsaturated and a decrease in mono- and di-unsaturated fatty acids. On the other hand, acute exercise inhibited PON activities while increasing PON3 protein content in liver microsomes and reversing the relative composition in mono-, di-, and poly-unsaturated fatty acids, suggesting that physical stress, by altering membrane composition, may impair PON release from liver membranes. In conclusion, we documented, for the first time, the presence of PON3 in rat serum and, notably, found that the upregulation of PON3, rather than PON1, appears to be associated with physical training.

【 授权许可】

Unknown   

  文献评价指标  
  下载次数:0次 浏览次数:1次