Magnesium Homeostasis in Myogenic Differentiation—A Focus on the Regulation of TRPM7, MagT1 and SLC41A1 Transporters

International Journal of Molecular Sciences

Laura Locatelli¹  Gian Vincenzo Zuccotti¹  Jeanette A. Maier¹  Monica Zocchi¹  Sara Castiglioni¹  Daniel Béchet²  André Mazur² 

[1]Department of Biomedical and Clinical Sciences Luigi Sacco, Università di Milano, 20157 Milano, Italy

[2]UNH—Unité de Nutrition Humaine, Université Clermont Auvergne, INRAE, F-63000 Clermont-Ferrand, France

关键词: myogenesis;    C2C12 cells;    magnesium;    TRPM7;    MagT1;    SLC41A1;   

DOI  :  10.3390/ijms23031658

来源: DOAJ

【 摘 要 】

Magnesium (Mg) is essential for skeletal muscle health, but little is known about the modulation of Mg and its transporters in myogenic differentiation. Here, we show in C2C12 murine myoblasts that Mg concentration fluctuates during their differentiation to myotubes, declining early in the process and reverting to basal levels once the cells are differentiated. The level of the Mg transporter MagT1 decreases at early time points and is restored at the end of the process, suggesting a possible role in the regulation of intracellular Mg concentration. In contrast, TRPM7 is rapidly downregulated and remains undetectable in myotubes. The reduced amounts of TRPM7 and MagT1 are due to autophagy, one of the proteolytic systems activated during myogenesis and essential for the membrane fusion process. Moreover, we investigated the levels of SLC41A1, which increase once cells are differentiated, mainly through transcriptional regulation. In conclusion, myogenesis is associated with alterations of Mg homeostasis finely tuned through the modulation of MagT1, TRPM7 and SLC41A1.

【 授权许可】

Unknown