Enzyme Immobilization Onto Biochar Produced by the Hydrothermal Carbonization of Biomass

Acta Chimica Slovenica

Željko Knez¹  Maja Leitgeb¹  Irena Pavlovič²  Mateja Primožič²  Gordana Hojnik Podrepšek²  Mojca Škerget² 

[1] University of Maribor, Faculty of Chemistry and Chemical EngineeringUniversity of Maribor, Faculty of Medicine;University of Maribor, Faculty of Chemistry and Chemical Engineering;

关键词: Hydrothermal carbonization;    biochar;    enzyme;    immobilization;    enzyme activity.;   

DOI  :  10.17344/acsi.2019.5013

来源: DOAJ

【 摘 要 】

For improving enzyme utilization in biotechnological processes, process costs have to be reduced, enzyme stability during industrial processes should be enhanced, and the recycle and reuse step should be favorable. The immobilization of enzymes is an important step for enhancing enzyme catalytic properties and operational stability. In order to reduce the costs of immobilization and consequently the cost of processes, a cheaper carrier (e.g. materials reclaimed as by-products) should be used. To achieve this, cellulase from Trichoderma sp. was immobilized on biochar obtained by low temperature hydrothermal carbonization (LTHTC) in two ways: by adsorption and by covalent binding via a crosslinking agent. The effect of immobilization time, enzyme concentration, type and concentration of the crosslinking agent and the types of carrier - biochar (LTHTC of waste from olive oil production (LTHTC of OL waste) or LTHTC of cellulose) on the immobilization efficiency and the residual activity of biocatalyst was studied. Higher immobilization efficiency and residual enzyme activity was achieved when the enzyme was covalently bound to biochar obtained by LTHTC of cellulose.

【 授权许可】

Unknown