Characterization of Glycosphingolipids in the Human Parathyroid and Thyroid Glands

International Journal of Molecular Sciences

Susann Teneberg¹  Chunsheng Jin¹  Karin Säljö²  Olov Norlén³  Peter Stålberg³  Anders Thornell⁴ 

[1] Department of Medical Biochemistry and Cell Biology, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, S-40530 Gothenburg, Sweden;Department of Plastic Surgery, Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, S-41345 Gothenburg, Sweden;Department of Surgical Sciences, Uppsala University Hospital, Uppsala University, S-75185 Uppsala, Sweden;Region Västra Götaland, Sahlgrenska University Hospital, S-41345 Gothenburg, Sweden;

关键词: thyroid glycosphingolipids;    parathyroid glycosphingolipids;    glycosphingolipid characterization;    mass spectrometry;    blood group antigens;   

DOI  :  10.3390/ijms22137044

来源: DOAJ

【 摘 要 】

As part of a systematic investigation of the glycosphingolipids in human tissues, acid and non-acid glycosphingolipids from human thyroid and parathyroid glands were isolated and characterized with mass spectrometry and binding of carbohydrate-recognizing ligands, with a focus on complex compounds. The glycosphingolipid patterns of the human parathyroid and thyroid glands were very similar. The major acid glycosphingolipids were sulfatide and the gangliosides GM3, GD3, GD1a, GD1b, GT1b and Neu5Ac-neolactotetraosylceramide, and the major non-acid glycosphingolipids were globotriaosylceramide and globoside. We also found neolactotetra- and neolactohexaosylceramide, the x₂ glycosphingolipid, and complex glycosphingolipids with terminal blood group O and A determinants in both tissues. A glycosphingolipid with blood group Le^b determinant was identified in the thyroid gland, and the parathyroid sample had a glycosphingolipid with terminal blood group B determinant. Immunohistochemistry demonstrated the expression of blood group A antigens in both the thyroid and parathyroid glands. A weak cytoplasmatic expression of the GD1a ganglioside was present in the thyroid, while the parathyroid gland had a strong GD1a expression on the cell surface. Thus, the glycosylation of human thyroid and parathyroid glands is more complex than previously appreciated. Our findings provide a platform for further studies of alterations of cell surface glycosphingolipids in thyroid and parathyroid cancers.

【 授权许可】

Unknown