Tissue Infiltrating LTi—Like Group 3 Innate Lymphoid Cells and T Follicular Helper Cells in Graves' and Hashimoto's Thyroiditis

Frontiers in Immunology

Guy Gorochov¹  Laurence Leenhardt¹  Outi Vaarala²  Robert Balderas³  Gaëll Mayer⁴  Gabrielle Deniziaut⁵  Makoto Miyara⁷  Delphine Abiven⁷  Laetitia Claër⁷  Natacha Monot⁸  Adeline Bauvois⁸  Audrey Mohr⁸  Muhammad Atif⁸  Isabelle Brocheriou⁹  Camille Buffet⁹  Rajneesh Malhotra^1,10  Christophe Trésallet^1,11 

[1] 0Sorbonne Université, Unité Thyroïde Tumeurs Endocrines, AP-HP Hôpital Pitié-Salpêtrière, Paris, France;BD Biosciences, San Diego, CA, United States;Clinical Development—Respiratory Inhalation and Oral Development, Global Medicines Development, AstraZeneca, Gothenburg, Sweden;D, AstraZeneca, Gothenburg, Sweden;Respiratory, Inflammation, and Autoimmunity, Medimmune, Gaithersburg, MD, United States;Service de Chirurgie Digestive, Bariatrique et Endocrinienne, Hôpital Avicenne, Bobigny, France;Sorbonne Université, Inserm, Centre d'immunologie et des maladies infectieuses—Paris (CIMI-PARIS), AP-HP Hôpital Pitié-Salpêtrière, Paris, France;Sorbonne Université, Inserm, Centre d'immunologie et des maladies infectieuses—Paris (CIMI-PARIS), Paris, France;Sorbonne Université, Service d'Anatomie Pathologique, AP-HP Hôpital Pitié-Salpêtrière, Paris, France;;Translational Science and Experimental Medicine, Early RIA, BioPharmaceuticals R&Université Paris Nord Seine St Denis Paris 13, Laboratoire d'imagerie Biomédicale (LIB) INSERM CNRS U678, CHU Pitié-Salpêtriẽre, Paris, France;

关键词: thyroid autoimmune disease;    regulatory T cells;    follicular helper T cells;    lymphoid tissue–inducer-like cells;    Hashimoto's thyroiditis;    Graves' disease;   

DOI  :  10.3389/fimmu.2020.00601

来源: DOAJ

【 摘 要 】

Background: Hashimoto's thyroiditis (HT) and Graves' disease (GD) are autoimmune thyroid disorders (AITDs). These conditions have been associated to abnormalities in circulating regulatory T cells (Tregs). We postulated that immune perturbations could be more pronounced at the thyroid tissue level.Methods: The phenotype of PBMCs and immune cells infiltrating thyroid tissue from 19 patients with HT, 21 patients with GD, and 30 controls has been analyzed by flow cytometry.Results: We report that blood and thyroid Treg cell subsets are similarly represented in all AITDs patients and controls. Increased Lymphoid tissue inducer (LTi)-like ILC3 and CXCR5+ PD-1hi CD4+ T follicular helper cells (Tfh) tissue-infiltrating cells, together with the prevalence of tertiary lymphoid structures (TLS) and germinal centers (GCs) represented a typical immune signature in all HT and 60% of GD patients. In the remaining group of GD patients, the absence of the aforementioned abnormalities was associated with a higher prevalence of ophthalmopathy.Conclusion: Tissue infiltrating Lymphoid Tissue inducer—like group 3 Innate Lymphoid cells and T follicular helper cells are increased in most thyroid autoimmune disease.

【 授权许可】

Unknown