Agarose and Its Derivatives as Supports for Enzyme Immobilization

Molecules

Enrico Sanjust¹  Roberto Fernandez-Lafuente²  Paolo Zucca³ 

[1]C/Marie Curie 2, Campus UAM-CSIC, Madrid 28049, Spain

[2]Departamento de Biocatalisis, ICP-CSIC

[3]Dipartimento di Scienze Biomediche, Università di Cagliari, 09042 Monserrato (CA), Italy

关键词: enzymes;    immobilization;    stabilization;    agar-agar;    agarose;    cross-linking;    functionalization;   

DOI  :  10.3390/molecules21111577

来源: DOAJ

【 摘 要 】

Agarose is a polysaccharide obtained from some seaweeds, with a quite particular structure that allows spontaneous gelation. Agarose-based beads are highly porous, mechanically resistant, chemically and physically inert, and sharply hydrophilic. These features—that could be further improved by means of covalent cross-linking—render them particularly suitable for enzyme immobilization with a wide range of derivatization methods taking advantage of chemical modification of a fraction of the polymer hydroxyls. The main properties of the polymer are described here, followed by a review of cross-linking and derivatization methods. Some recent, innovative procedures to optimize the catalytic activity and operational stability of the obtained preparations are also described, together with multi-enzyme immobilized systems and the main guidelines to exploit their performances.

【 授权许可】

Unknown